水稻（Oryza sativa）幼茎对硝酸盐的高亲和力摄取。

High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles.

机构信息

Graduate School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.

出版信息

J Plant Res. 2011 Mar;124(2):305-9. doi: 10.1007/s10265-010-0375-9. Epub 2010 Sep 23.

DOI:10.1007/s10265-010-0375-9

PMID:20862512

Abstract

Nitrate uptake by rice coleoptiles was evaluated using ¹⁵N-nitrate in relation to the expression of high-affinity nitrate uptake-related genes, OsNRT2s (OsNRT2.1-2.4) and OsNAR2s (OsNAR2.1 and 2.2). Apparent nitrate uptake by coleoptiles was about one-sixth of that by hydroponically cultured seedling roots. Real-time RT-PCR analysis revealed that OsNRT2.1, a root-specific key gene of inducible high-affinity transport system for nitrate, was most strongly induced in coleoptiles following nitrate supply initiation, while other OsNRT2s and OsNAR2s showed modest induction. These results suggest that rice coleoptiles may have high-affinity transport systems for nitrate similar to roots, and can be model organs for nutrient uptake by submerged plant shoots.

摘要

采用¹⁵N-硝酸盐评估了水稻芽鞘对硝酸盐的摄取，同时还研究了高亲和力硝酸盐摄取相关基因 OsNRT2s（OsNRT2.1-2.4）和 OsNAR2s（OsNAR2.1 和 2.2）的表达情况。芽鞘对硝酸盐的表观摄取量约为水培幼苗根系的六分之一。实时 RT-PCR 分析表明，根特异性关键基因 OsNRT2.1 是诱导型高亲和力硝酸盐转运系统的关键基因，在硝酸盐供应起始后，芽鞘中的表达受到强烈诱导，而其他 OsNRT2s 和 OsNAR2s 的表达则受到适度诱导。这些结果表明，水稻芽鞘可能具有类似于根的高亲和力硝酸盐转运系统，并且可以作为水生植物芽吸收养分的模型器官。