Bois-Joyeux B, Chanez M, Peret J
Centre de Recherche sur la Nutrition, CNRS, Meudon, France.
Diabete Metab. 1990 Dec;16(6):504-12.
Food intake, plasma glucose, insulin (I) and glucagon (G), hepatic glycogen and fructose 2,6-bisphosphate (F-2, 6-P2) and liver glucokinase, glucose 6-phosphatase (G6-Pase), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6-PF-2 kinase/F-2, 6-P2ase), pyruvate kinase (PK-L) and phosphoenolpyruvate carboxykinase (PEPCK) activities were measured in 2 and 22-month-old rats before 3 d starvation and after 2, 4, 6, 24 and 48 h refeeding a high carbohydrate (HC, 74% w/w) diet. Expressed per 100 g of body weight, the food intake of old rats was 55% lower than that of young rats and the amount of carbohydrate absorbed hourly during the first 6 h of refeeding was 2.4-fold higher in young than in old rats. During the first 6 h of refeeding plasma glucose increased 2-fold and returned to normal values after 24 h in young rats, while plasma glucose did not change during refeeding in old rats. In young rats [I] fell by 85% after starvation and returned to normal values 2 h after refeeding. [I] was higher in old than in young rats; it decreased by 40% after starvation and returned to the basal value 4 h after refeeding. No marked changes were observed in plasma [G] in both groups. No difference was observed in hepatic glycogen in the two groups, while F-2, 6-P2 was higher in old than in young rats. In young rats, the opposite changes in liver glucokinase and G6-Pase activities occurring after starvation and during refeeding were
在2月龄和22月龄大鼠3天饥饿前以及重新喂食高碳水化合物（HC，74% w/w）饮食2、4、6、24和48小时后，测量其食物摄入量、血浆葡萄糖、胰岛素（I）和胰高血糖素（G）、肝糖原、果糖2,6-二磷酸（F-2,6-P2）以及肝葡萄糖激酶、葡萄糖6-磷酸酶（G6-Pase）、6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶（6-PF-2激酶/F-2,6-P2酶）、丙酮酸激酶（PK-L）和磷酸烯醇式丙酮酸羧激酶（PEPCK）的活性。以每100克体重计算，老年大鼠的食物摄入量比幼年大鼠低55%，重新喂食的前6小时内，幼年大鼠每小时吸收的碳水化合物量比老年大鼠高2.4倍。在重新喂食的前6小时，幼年大鼠的血浆葡萄糖增加了2倍，并在24小时后恢复到正常值，而老年大鼠在重新喂食期间血浆葡萄糖没有变化。在幼年大鼠中，饥饿后[I]下降了85%，重新喂食2小时后恢复到正常值。老年大鼠的[I]高于幼年大鼠；饥饿后下降了40%，重新喂食4小时后恢复到基础值。两组的血浆[G]均未观察到明显变化。两组的肝糖原没有差异，而老年大鼠的F-2,6-P2高于幼年大鼠。在幼年大鼠中，饥饿后和重新喂食期间肝葡萄糖激酶和G6-Pase活性发生了相反的变化。
