Tuan Pham Anh, Park Nam Il, Li Xiaohua, Xu Hui, Kim Haeng Hoon, Park Sang Un
Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-764, Korea.
J Agric Food Chem. 2010 Oct 27;58(20):10911-7. doi: 10.1021/jf1021384. Epub 2010 Sep 23.
The cDNAs encoding phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) were cloned from garlic (Allium sativum) using reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers and 5' and 3' rapid amplification of cDNA ends (RACE) PCR. Amino acid sequence alignments showed that AsPAL and AsC4H have more than 70% amino acid identity with their homologues in other plants. The expression of AsPAL and AsC4H transcripts was highest in the roots but surprisingly low in the bulbils, where phenylpropanoid compounds are most concentrated. These results suggest that some phenylpropanoids are synthesized in the roots and subsequently transported to the bulbils of A. sativum .
使用简并引物通过逆转录聚合酶链反应（RT-PCR）以及5'和3' cDNA末端快速扩增（RACE）PCR，从大蒜（Allium sativum）中克隆了编码苯丙氨酸解氨酶（PAL）和肉桂酸4-羟化酶（C4H）的cDNA。氨基酸序列比对表明，AsPAL和AsC4H与其在其他植物中的同源物具有70%以上的氨基酸同一性。AsPAL和AsC4H转录本的表达在根中最高，但令人惊讶的是在珠芽中很低，而苯丙烷类化合物在珠芽中最为集中。这些结果表明，一些苯丙烷类化合物是在根中合成的，随后运输到大蒜的珠芽中。
