Bligh J, Schramm G, Chiodini P L, Doenhoff M J
Department of Clinical Parasitology, Hospital for Tropical Diseases, Mortimer Market Centre, London, UK.
Ann Trop Med Parasitol. 2010 Sep;104(6):511-20. doi: 10.1179/136485910X12786389891245.
A serological assay was developed to assess the outcome of the treatment of intestinal schistosomiasis with praziquantel, in patients with Schistosoma mansoni infection. Each of 33 patients (seven found to be excreting S. mansoni eggs and 26 egg-negatives found seropositive for antibodies against antigens from S. mansoni eggs) had two to five serum specimens assayed, the sera being collected at the time of diagnosis and at least once after praziquantel treatment. The sera were tested in ELISA against three antigen preparations: the unfractionated soluble egg antigens of S. margrebowiei and S. mansoni (SmSEA) and a cationic antigen fraction (CEF6) purified from the SmSEA. The dynamics of the post-treatment antibody levels were variable. In a minority of the patients, antibody levels declined relatively rapidly (within 5-12 months), to ELISA negativity, with the levels of the anti-CEF6 antibodies declining more rapidly than those of the anti-SmSEA antibodies. In the remaining patients, however, the levels of these specific antibodies declined only slowly or not at all over a 2- to 3-year period. The post-treatment monitoring of the levels of anti-schistosome-egg antibodies, particularly those of anti-CEF6 antibodies, may help to distinguish the treatments that result in parasitological cure from those that are only partially successful.
开发了一种血清学检测方法，用于评估曼氏血吸虫感染患者使用吡喹酮治疗肠道血吸虫病的疗效。33例患者（7例粪便中发现曼氏血吸虫虫卵，26例粪便虫卵阴性但血清中抗曼氏血吸虫虫卵抗原抗体呈阳性）每人检测了2至5份血清样本，血清在诊断时以及吡喹酮治疗后至少采集一次。血清通过酶联免疫吸附测定法（ELISA）检测三种抗原制剂：未分级的Margrebowiei血吸虫和曼氏血吸虫可溶性虫卵抗原（SmSEA）以及从SmSEA中纯化的阳离子抗原组分（CEF6）。治疗后抗体水平的动态变化各不相同。少数患者抗体水平相对较快下降（5至12个月内）至ELISA阴性，抗CEF6抗体水平比抗SmSEA抗体下降得更快。然而，在其余患者中，这些特异性抗体水平在2至3年期间仅缓慢下降或根本未下降。治疗后监测抗血吸虫虫卵抗体水平，特别是抗CEF6抗体水平，可能有助于区分导致寄生虫学治愈的治疗方法和仅部分成功的治疗方法。
