Doenhoff M J, Wheeler J G, Tricker K, Hamilton J V, Sturrock R F, Butterworth A E, Ouma J H, Mbugua G G, Kariuki C, Koech D
School of Biological Sciences, University of Wales Bangor, Deiniol Road, Bangor LL57 2UW, UK.
Ann Trop Med Parasitol. 2003 Oct;97(7):697-709. doi: 10.1179/000349803225002354.
Circulating IgG antibody reactivity and excreted egg counts were investigated in 489 Kenyans given chemotherapy for schistosomiasis mansoni. Antibody reactivity was measured in ELISA, using either unfractionated aqueous soluble constituents of Schistosoma mansoni eggs (SEA) or CEF6 (a soluble fraction of S. mansoni eggs containing two cationic antigens) as the antigen source. Antibody reactivity for each antigen source was strongly associated with egg counts, both pre- and post-treatment. Approximately 6 months after chemotherapy, egg counts were zero in 84% of the subjects. The mean optical densities (OD) measured in the post-treatment ELISA were 60% (CEF6) or 45% (SEA) lower than the pre-treatment values, the reduction in the OD with CEF6 as antigen source being significantly greater than that observed with SEA (P <0.001). The usefulness of an assay for antibody reactivity in monitoring the effects of the treatment of schistosomiasis is discussed.
对489名接受曼氏血吸虫病化疗的肯尼亚人进行了循环IgG抗体反应性和粪便虫卵计数研究。采用酶联免疫吸附测定法（ELISA）测量抗体反应性，抗原来源为曼氏血吸虫卵的未分级水溶性成分（SEA）或CEF6（曼氏血吸虫卵的一种可溶性成分，含有两种阳离子抗原）。治疗前后，每种抗原来源的抗体反应性均与虫卵计数密切相关。化疗后约6个月，84%的受试者粪便虫卵计数为零。治疗后ELISA检测的平均光密度（OD）比治疗前值低60%（CEF6）或45%（SEA），以CEF6作为抗原来源时OD的降低显著大于以SEA作为抗原来源时（P<0.001）。本文讨论了抗体反应性检测在监测血吸虫病治疗效果方面的实用性。
