Lipid Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA.
Atherosclerosis. 2010 Nov;213(1):251-5. doi: 10.1016/j.atherosclerosis.2010.02.041. Epub 2010 Mar 9.
We evaluated direct low density lipoprotein (LDL) cholesterol (C) and high density lipoprotein (HDL) cholesterol (C) versus standard methods using fasting plasma samples from participants in cycle 6 of the Framingham Offspring Study.
Direct LDL-C and HDL-C measurements were performed on fasting plasma from male (1335 controls, 173 CHD cases) and female (1606 controls, 74 cases) participants, and compared with LDL-C, as calculated with the Friedewald formula, and HDL-C, as measured after dextran-Mg(2+) precipitation.
Values for direct LDL-C and HDL-C correlated well with standard methods (both about r(2)=0.94, p<0.001) with similar absolute values. Biases of >10% were present for 7.7% of samples for LDL-C, while for HDL-C this value was 8.5%. Despite higher use of cholesterol-lowering medication in CHD cases, calculated or direct LDL-C values were still well above recommended values [<2.6 mmol/L (100 mg/dL)] in CHD cases, especially in females.
Direct assays for both LDL-C and HDL-C provide an acceptable guide for lipid treatment. In Framingham Offspring Study participants most CHD cases had LDL-C levels above the recommended target.
我们评估了直接低密度脂蛋白（LDL）胆固醇（C）和高密度脂蛋白（HDL）胆固醇（C）与使用弗雷明汉后代研究第 6 周期参与者的空腹血浆样本的标准方法。
对男性（1335 名对照者，173 名 CHD 病例）和女性（1606 名对照者，74 名病例）参与者的空腹血浆进行直接 LDL-C 和 HDL-C 测量，并与 LDL-C（用 Friedewald 公式计算）和 HDL-C（用葡聚糖-Mg(2+)沉淀后测量）进行比较。
直接 LDL-C 和 HDL-C 值与标准方法相关性良好（均约 r(2)=0.94，p<0.001），绝对值相似。LDL-C 有 7.7%的样本偏差>10%，而 HDL-C 为 8.5%。尽管 CHD 病例中使用了更多的降胆固醇药物，但计算或直接 LDL-C 值仍远高于推荐值[<2.6 mmol/L（100 mg/dL）]，尤其是女性。
直接 LDL-C 和 HDL-C 测定值为脂质治疗提供了可接受的指导。在弗雷明汉后代研究参与者中，大多数 CHD 病例的 LDL-C 水平高于推荐目标。
