Lan Bing, Chen Peng, Jiri Mutu, He Na, Feng Tian, Liu Kai, Jin Tianbo, Kang Longli
Key Laboratory for Basic Life Science Research of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, #6 East Wenhui Road, Xianyang, 712082, Shaanxi, China.
Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, 712082, Shaanxi, China.
Rheumatol Int. 2016 Mar;36(3):405-12. doi: 10.1007/s00296-015-3378-y. Epub 2015 Oct 5.
Current evidence suggests heredity and metabolic syndrome contributes to gout progression. Specifically, the WDR1 and CLNK genes may play a role in gout progression in European ancestry populations. However, no studies have focused on Chinese populations, especially Tibetan individuals. This study aims to determine whether variations in these two genes correlate with gout-related indices in Chinese-Tibetan gout patients. Eleven single-nucleotide polymorphisms in the WDR1 and CLNK genes were detected in 319 Chinese-Tibetan gout patients and 318 controls. We used one-way analysis of variance to evaluate the polymorphisms' effects on gout based on mean serum levels of metabolism indicators, such as albumin, glucose (GLU), triglycerides, cholesterol, high-density lipoproteins (HDL-C), creatinine, and uric acid, from fasting venous blood samples. All p values were Bonferroni corrected. Polymorphisms of the WDR1 and CLNK genes affected multiple risk factors for gout development. Significant differences in serum GLU levels were detected between different genotypic groups with WDRI polymorphisms rs4604059 (p = 0.005) and rs12498927 (p = 0.005). In addition, significant differences in serum HDL-C levels were detected between different genotypic groups with the CLNK polymorphism rs2041215 (p = 0.001). Polymorphisms of CLNK also affected levels of albumin, triglycerides, and creatinine. This study is the first to investigate and identify positive correlations between WDR1 and CLNK gene polymorphisms in Chinese-Tibetan populations. Our findings provide significant evidence for the effect of genetic polymorphisms on gout-related factors in Chinese-Tibetan populations.
目前的证据表明，遗传因素和代谢综合征会促使痛风病情发展。具体而言，WDR1和CLNK基因可能在欧洲血统人群的痛风病情发展中发挥作用。然而，尚无研究聚焦于中国人群，尤其是藏族个体。本研究旨在确定这两个基因的变异是否与中国藏族痛风患者的痛风相关指标相关。在319名中国藏族痛风患者和318名对照者中检测了WDR1和CLNK基因中的11个单核苷酸多态性。我们采用单因素方差分析，根据空腹静脉血样本中白蛋白、葡萄糖（GLU）、甘油三酯、胆固醇、高密度脂蛋白（HDL-C）、肌酐和尿酸等代谢指标的平均血清水平，评估这些多态性对痛风的影响。所有p值均经Bonferroni校正。WDR1和CLNK基因的多态性影响痛风发展的多个风险因素。在具有WDRI多态性rs4604059（p = 0.005）和rs12498927（p = 0.005）的不同基因型组之间，检测到血清GLU水平存在显著差异。此外，在具有CLNK多态性rs2041215的不同基因型组之间，检测到血清HDL-C水平存在显著差异（p = 0.001）。CLNK的多态性也影响白蛋白、甘油三酯和肌酐的水平。本研究首次调查并确定了中国藏族人群中WDR1和CLNK基因多态性之间的正相关关系。我们的研究结果为基因多态性对中国藏族人群痛风相关因素的影响提供了重要证据。
