通过使用同裂酶NdeII-Sau3AI处理，在人类染色体着丝粒异染色质区域之间发现了新的差异。
New distinctions between regions of centromeric heterochromatin in human chromosomes by treatments with the isoschizomers NdeII-Sau3AI.
作者信息
Ludenã P, Sentís C, Fernández-Peralta A M, Gonzalez-Aguilera J, Fernández-Piqueras J
机构信息
Departamento de Biologia, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
出版信息
Genome. 1990 Dec;33(6):785-8. doi: 10.1139/g90-117.
The isoschizomers NdeII-Sau3AI (decreases GATC) have been used to characterize heterochromatic regions in human chromosomes. The findings with NdeII are identical with those previously published with MboI, but the results with Sau3AI provide evidence for new distinctions of centromeric heterochromatin in chromosomes 5 and 6. The results are discussed in relation to the chromatin organization at these regions an the mechanisms of the action of restriction endonucleases.
同裂酶NdeII - Sau3AI（切割GATC序列，切割频率降低）已被用于鉴定人类染色体中的异染色质区域。NdeII的研究结果与之前发表的MboI的研究结果一致，但Sau3AI的结果为5号和6号染色体着丝粒异染色质的新差异提供了证据。本文结合这些区域的染色质组织以及限制性内切酶的作用机制对结果进行了讨论。
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