一种利用限制性核酸内切酶识别人类染色体异染色质区域的新方法。
A new approach in recognition of heterochromatic regions of human chromosomes by means of restriction endonucleases.
作者信息
Babu A, Agarwal A K, Verma R S
机构信息
Division of Medical Genetics, Beth Israel Medical Center, Mount Sinai School of Medicine, New York.
出版信息
Am J Hum Genet. 1988 Jan;42(1):60-5.
The heteromorphisms of C-band regions of human chromosomes are evaluated by means of restriction endonucleases AluI, DdeI, MboI, and RsaI. Every chromosome exhibits heteromorphic markers of the C-band regions except chromosome 8. Each enzyme was found to be highly characteristic in its staining profile, a result that clearly suggests the diversity of heterochromatin. The inherent C-band-region heterochromatin variability that is revealed by these enzymes provides a valuable tool in identifying markers as compared with other previously described techniques.
利用限制性内切酶AluI、DdeI、MboI和RsaI对人类染色体C带区域的异态性进行评估。除8号染色体外，每条染色体均表现出C带区域的异态性标记。发现每种酶在其染色图谱中都具有高度特征性，这一结果清楚地表明了异染色质的多样性。与其他先前描述的技术相比，这些酶所揭示的固有C带区域异染色质变异性为识别标记提供了一种有价值的工具。
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