Molecular, Cellular, and Developmental Biology, 347 UCB, University of Colorado, Boulder, CO 80309, USA.
Trends Genet. 2010 Nov;26(11):468-75. doi: 10.1016/j.tig.2010.08.005. Epub 2010 Sep 23.
Transcription factor interactions with DNA are one of the primary mechanisms by which expression is modulated, yet their evolution remains poorly understood. Chromatin immunoprecipitation followed by microarray (ChIP-chip) or sequencing (ChIP-Seq) has revolutionized the study of protein-DNA interactions. However, only recently has attention focused on determining to what extent these regulatory interactions vary between species across entire genomes. A series of recent studies have compared in vivo binding data across a range of evolutionary distances. Binding events diverge rapidly, indicating gene regulation is an evolutionarily flexible process.
转录因子与 DNA 的相互作用是调节表达的主要机制之一，但它们的进化仍知之甚少。染色质免疫沉淀结合微阵列（ChIP-chip）或测序（ChIP-Seq）技术极大地推动了蛋白质-DNA 相互作用的研究。然而，直到最近，人们才开始关注这些调控相互作用在整个基因组中在物种间的差异程度。一系列最近的研究比较了一系列进化距离的体内结合数据。结合事件迅速分化，表明基因调控是一个进化灵活的过程。
