Department of Dermatology, Ernst Moritz Arndt University, Greifswald, Germany.
Am J Infect Control. 2011 Mar;39(2):98-103. doi: 10.1016/j.ajic.2010.05.026.
Surgical gloves provide a protective barrier for patients and members of the surgical team. Although glove integrity is important in an era of blood-borne pathogens, little data exist on bacterial passage after glove perforation. This study evaluated the impact of antimicrobial surgical gloves in reducing microbial passage after glove puncture in a model of wound contamination.
Staphylococcus aureus (ATCC 6538) and Brevundimonas diminuta (DSM 1639) were used to prepare a standardized suspension for testing bacterial passage after glove puncture in volunteers wearing single-layer gloves (group A), double-layer gloves (group B), or antimicrobial trilayer gloves (group C). After exposure periods of 5, 10, 30 and 45 minutes, the outer test gloves were removed and microbial passage was measured on the inner surface of the base gloves. Multiple repetitions (5 or 6) were performed at each sampling time.
Microbial passage at 5-, 10-, 30-, and 45-minute exposures were analyzed both separately and combined (5 and 10 minutes and 30 and 45 minutes). No difference was observed in microbial passage between group A and group B at the 10-, 30-, and 45-minute exposures for S aureus, whereas a significant reduction in microbial passage was observed in group C compared with group A (P ≤ .05 to < .005) at the 5-, 30-, and 45-minute exposures for both S aureus and B diminuta. When timed groups were combined (5 and 10 minutes and 30 and 45 minutes), a significant reduction (P ≤ .01 to ≤ .005) in microbial passage of S aureus and B diminuta was observed in group C compared with both group A and group B.
These findings represent the first evidence that microbial passage across surgical gloves can be reduced significantly using an innovative antimicrobial glove technology.
手术手套为患者和手术团队成员提供了一道保护屏障。虽然在血源性病原体时代，手套完整性很重要，但手套穿孔后细菌穿透的数据很少。本研究评估了抗菌手术手套在污染伤口模型中减少手套穿孔后微生物穿透的影响。
使用金黄色葡萄球菌（ATCC 6538）和寡养单胞菌（DSM 1639）制备标准化悬浮液，用于测试志愿者在单层手套（A 组）、双层手套（B 组）或抗菌三层手套（C 组）下穿孔后手套的细菌穿透。暴露 5、10、30 和 45 分钟后，取出外层测试手套，测量基础手套内表面的微生物穿透。在每个采样时间重复多次（5 或 6 次）。
分别和组合（5 分钟和 10 分钟以及 30 分钟和 45 分钟）分析了 5、10、30 和 45 分钟暴露时的微生物穿透。在 10、30 和 45 分钟暴露时，A 组和 B 组之间的微生物穿透没有差异，但与 A 组相比，C 组在 5、30 和 45 分钟暴露时的微生物穿透显著减少（P≤.05 至<.005）金黄色葡萄球菌和 B diminuta。当组合定时组（5 分钟和 10 分钟以及 30 分钟和 45 分钟）时，与 A 组和 B 组相比，C 组金黄色葡萄球菌和 B diminuta 的微生物穿透显著减少（P≤.01 至≤.005）。
这些发现首次证明，使用创新的抗菌手套技术可以显著减少手术手套的微生物穿透。
