Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
Allergol Int. 2010 Dec;59(4):399-408. doi: 10.2332/allergolint.10-OA-0218. Epub 2010 Sep 25.
IL-25, which is a member of the IL-17 family, induces Th2 cell differentiation and Th2 cytokine production, contributing to induction of Th2-type immune responses and diseases, as a result of which it suppresses Th1- and Th17-type immune responses.
To elucidate the role of IL-25 in the pathogenesis of IL-17-mediated delayed-type hypersensitivity (DTH), IL-25-deficient mice were sensitized with methylated BSA (mBSA), and then a DTH reaction was induced by mBSA challenge. mBSA-specific T-cell induction was assessed on the basis of cell proliferation and cytokine production. The DTH reaction was evaluated on the basis of tissue swelling, histology and inflammatory mediator expression.
IL-25 expression was markedly reduced in local DTH lesions. However, mBSA-specific Th1, Th2 and Th17 cell induction, and the mBSA-induced DTH reaction were comparable in IL-25-deficient and wild-type mice.
IL-25 is not essential for differentiation of Th1, Th2 and Th17 cells in the sensitization phase or induction of local inflammation in the elicitation phase of the mBSA-induced DTH reaction.
IL-25 是 IL-17 家族的一员，可诱导 Th2 细胞分化和 Th2 细胞因子产生，导致 Th2 型免疫应答和疾病的发生，从而抑制 Th1 和 Th17 型免疫应答。
为了阐明 IL-25 在 IL-17 介导的迟发型超敏反应（DTH）发病机制中的作用，用甲基化 BSA（mBSA）对 IL-25 缺陷型小鼠进行致敏，然后用 mBSA 攻击诱导 DTH 反应。基于细胞增殖和细胞因子产生来评估 mBSA 特异性 T 细胞的诱导。根据组织肿胀、组织学和炎症介质表达来评估 DTH 反应。
IL-25 在局部 DTH 病变中的表达明显降低。然而，在 IL-25 缺陷型和野生型小鼠中，mBSA 特异性 Th1、Th2 和 Th17 细胞的诱导以及 mBSA 诱导的 DTH 反应并无差异。
在 mBSA 诱导的 DTH 反应的致敏阶段或激发阶段，IL-25 对于 Th1、Th2 和 Th17 细胞的分化或局部炎症的诱导并非必需。
