Department of Ecology, Institute for Biological Research Siniša Stanković, University of Belgrade, Bulevar Despota Stefana 142, Belgrade, Serbia.
Immunobiology. 2011 Jul;216(7):763-70. doi: 10.1016/j.imbio.2010.12.007. Epub 2010 Dec 25.
Contact hypersensitivity (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. Majority of CHS studies were conducted in mice and there is paucity of data in other experimental animals. In the present study, characteristics of contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) were determined in Th1-prone Dark Agouti (DA) rats by evaluating sensitization phase as a function of time-dependent changes in draining lymph nodes (DLN). Apart from basic indices of DLN activity (cellularity and proliferation), the production of cytokines relevant for CHS induction, interleukin-6 (IL-6), interferon-γ (IFN-γ), interleukin-17 (IL-17) and interleukin-4 (IL-4) was analyzed. Anti-inflammatory cytokine interleukin-10 (IL-10) production by DLN cells was determined as well. Highest production of IL-6, IFN-γ and IL-17 in sensitized animals was observed at day 3 after DNCB application, with a decrease at day 5. Increased messages for IFN-γ and IL-17 were noted at this time point. In contrast to inflammatory cytokines, anti-inflammatory cytokine interleukin-4 (IL-4) was undetectable during the entire sensitization phase. Differential pattern (IL-6 and IFN-γ) and level (IFN-γ and IL-17) of inflammatory cytokine production was noted in sensitized Th2-prone Albino Oxford (AO) rats. Similarly to DA rats, no changes in IL-4 were noted in AO rats. Strain-dependent differences in inflammatory cytokine production seem to be based on anti-inflammatory cytokine interleukin-10 (IL-10). Production of IFN-γ concomitantly with undetectable IL-4 in both strains classify rat CHS to DNCB as Th1/type 1 reaction. Detection of IL-17 in sensitized DLN cells points to the involvement of T(IL-17) cells in rat contact hypersensitivity.
接触超敏反应 (CHS) 是一种 T 细胞介导的皮肤炎症反应，由皮肤接触小敏化化学物质（半抗原）引起。大多数 CHS 研究都是在小鼠中进行的，而在其他实验动物中数据很少。在本研究中，通过评估引流淋巴结 (DLN) 随时间变化的功能，确定了倾向于 Th1 的 Dark Agouti (DA) 大鼠对二硝基氯苯 (DNCB) 的接触超敏反应特征。除了 DLN 活性的基本指标（细胞数和增殖）外，还分析了与 CHS 诱导相关的细胞因子的产生，包括白细胞介素-6 (IL-6)、干扰素-γ (IFN-γ)、白细胞介素-17 (IL-17) 和白细胞介素-4 (IL-4)。还测定了 DLN 细胞产生的抗炎细胞因子白细胞介素-10 (IL-10)。在 DNCB 应用后第 3 天，致敏动物的 IL-6、IFN-γ 和 IL-17 产生量最高，第 5 天下降。此时观察到 IFN-γ 和 IL-17 的表达增加。与炎症细胞因子相反，在整个致敏阶段均未检测到抗炎细胞因子白细胞介素-4 (IL-4)。在倾向于 Th2 的 Albino Oxford (AO) 大鼠中，观察到炎症细胞因子产生的差异模式（IL-6 和 IFN-γ）和水平（IFN-γ 和 IL-17）。与 DA 大鼠一样，在 AO 大鼠中也未观察到 IL-4 的变化。炎症细胞因子产生的种系差异似乎基于抗炎细胞因子白细胞介素-10 (IL-10)。两种品系中 IFN-γ 的产生与 IL-4 的未检测到同时存在，将大鼠对 DNCB 的 CHS 分类为 Th1/1 型反应。在致敏的 DLN 细胞中检测到 IL-17 表明 T(IL-17)细胞参与了大鼠接触超敏反应。
