Kimber I, Kinnaird A, Peters S W, Mitchell J A
ICI Central Toxicology Laboratory, Alderley Park, Macclesfield, UK.
Int Arch Allergy Appl Immunol. 1990;93(1):47-53. doi: 10.1159/000235278.
We have investigated whether there exists a correlation between the induction of draining lymph node cell (LNC) proliferation in contact allergy and the accumulation of dendritic cells (DC) within such lymph nodes. CBA/Ca mice, which compared with mice of BALB/c strain, mount a more vigorous lymphocyte proliferative response following sensitization, also exhibited a more marked accumulation of DC in draining lymph nodes 24 h following skin painting. Moreover, studies with the skin-sensitizing fluorochromes fluorescein isothiocyanate (FITC) and rhodamine B isothiocyanate (RITC) revealed that DC-enriched fractions of draining LNC prepared from CBA/Ca mice contained a higher percentage of antigen-bearing cells than did those from BALB/c mice. A relationship between DC migration into lymph nodes and the magnitude of the induced LNC proliferative response was also indicated by experiments performed in BALB/c mice with a variety of contact allergens. It was observed that there was a direct correlation between the vigour of the proliferative response measured 3 days following exposure and the frequency of DC in draining nodes at 24 h. Collectively these data suggest that following skin sensitization the migration of DC into the draining lymph nodes influences quantitatively the primary immune response and the development of contact allergy.
我们研究了接触性过敏中引流淋巴结细胞（LNC）增殖的诱导与此类淋巴结内树突状细胞（DC）的积聚之间是否存在相关性。与BALB/c品系小鼠相比，CBA/Ca小鼠在致敏后会产生更强烈的淋巴细胞增殖反应，在皮肤涂抹后24小时，其引流淋巴结中DC的积聚也更为明显。此外，对皮肤致敏荧光染料异硫氰酸荧光素（FITC）和异硫氰酸罗丹明B（RITC）的研究表明，从CBA/Ca小鼠制备的引流LNC中富含DC的部分比从BALB/c小鼠制备的含有更高百分比的携带抗原细胞。在BALB/c小鼠中使用多种接触性变应原进行的实验也表明了DC迁移至淋巴结与诱导的LNC增殖反应强度之间的关系。观察到暴露后3天测量的增殖反应强度与24小时引流淋巴结中DC的频率之间存在直接相关性。这些数据共同表明，皮肤致敏后DC迁移至引流淋巴结会定量影响初次免疫反应和接触性过敏的发展。
