Roncari D A
Department of Medicine, University of Toronto, Ontario, Canada.
Int J Obes. 1990;14 Suppl 3:187-92.
Adipose cells grown in sub-culture are useful to elucidate genetic factors in obesity. Most omental adipose cell strains from 140 massively obese (greater than 170 percent of reference body weight) subjects replicated, in successive sub-cultures, to a significantly higher degree than cells from lean or moderately obese persons. The difference was due to a greater number of rapidly dividing clones. Adipose cells from the massively obese related into the culture medium proteins, native Mr 20,000-65,000, mitogenic on rat preadipocytes. Mitogenic activity of the medium was much less evident with cells from the lean. In the case of several cell strains, culture with 17-beta-estradiol increased the mitogenic activity of the medium. Omental adipose tissue of the massive obese also contained a greater number of adipose cell clones susceptible to differentiation. Hybrids of adipose cells from the massively obese fused with murine renal adenocarcinoma cells (RAG) revealed more prominent differentiation than hybrids comprised of adipose cells from the lean. Further, only those comprised of adipose cells from the obese could recapitulate differentiation in sub-cultures. These findings in culture probably reflect major heritable factors that facilitate the development of massive obesity in humans.
传代培养的脂肪细胞有助于阐明肥胖中的遗传因素。来自140名极度肥胖（超过参考体重的170%）受试者的大多数网膜脂肪细胞系在连续传代培养中，比来自瘦人或中度肥胖者的细胞复制程度明显更高。这种差异是由于快速分裂的克隆数量更多。来自极度肥胖者的脂肪细胞分泌到培养基中的蛋白质，天然分子量为20,000 - 65,000，对大鼠前脂肪细胞有促有丝分裂作用。瘦人细胞的培养基促有丝分裂活性则不太明显。对于几种细胞系，用17-β-雌二醇培养可增加培养基的促有丝分裂活性。极度肥胖者的网膜脂肪组织中也含有更多易于分化的脂肪细胞克隆。来自极度肥胖者的脂肪细胞与鼠肾腺癌细胞（RAG）融合的杂交体比由瘦人脂肪细胞组成的杂交体表现出更显著的分化。此外，只有那些由肥胖者脂肪细胞组成的杂交体才能在传代培养中重现分化。培养中的这些发现可能反映了促进人类极度肥胖发展的主要遗传因素。
