Bidló-Iglóy M
Institute for Drug Research, Budapest, Hungary.
J Chromatogr. 1990 Nov 9;520:367-71. doi: 10.1016/0021-9673(90)85121-b.
Ion-pair reversed-phase high-performance liquid chromatography proved to be useful for the separation of basic compounds of similar structure. Using suitable ion-pairing reagent concentration, pH and ionic strength values, good separations can be achieved. An ion-pair chromatographic study of a benzodioxanyl derivative, an aminopyrimidine derivative and a lysergic acid derivative was performed. Each of these compounds may be contaminated by a structural isomer produced as a by-product during the synthesis. By choosing the optimum experimental conditions these isomers can be separated from each other and from other related compounds and can be quantified in various substances and pharmaceutical products.
离子对反相高效液相色谱法被证明可用于分离结构相似的碱性化合物。通过使用合适的离子对试剂浓度、pH值和离子强度值，可以实现良好的分离效果。对一种苯并二氧杂环戊烯基衍生物、一种氨基嘧啶衍生物和一种麦角酸衍生物进行了离子对色谱研究。这些化合物中的每一种都可能被合成过程中作为副产物产生的结构异构体污染。通过选择最佳实验条件，这些异构体可以相互分离，并与其他相关化合物分离，还可以在各种物质和药品中进行定量分析。
