Watkins P J, Gorrod J W
Chelsea Department of Pharmacy, King's College, University of London, UK.
J Chromatogr. 1993 Jun 23;616(1):79-85. doi: 10.1016/0378-4347(93)80474-i.
Reversed-phase ion-pair high-performance liquid chromatography has been investigated for the separation, detection, identification and quantitation of the isomeric 1N- and 3N-oxide metabolites of metoprine, pyrimethamine and trimethoprim. A rapid and sensitive analytical method for the simultaneous determination of the isomeric 1N- and 3N-oxides of metoprine and pyrimethamine was devised for in vitro metabolic studies by optimisation of mobile phase pH, pairing-ion concentration, secondary-ion concentration and percentage organic modifier.
已对反相离子对高效液相色谱法进行了研究，用于分离、检测、鉴定和定量测定甲氧苄啶、乙胺嘧啶和甲氧苄氨嘧啶的1N-和3N-氧化物同分异构体代谢物。通过优化流动相pH值、配对离子浓度、二次离子浓度和有机改性剂百分比，设计了一种快速灵敏的分析方法，用于体外代谢研究中同时测定甲氧苄啶和乙胺嘧啶的1N-和3N-氧化物同分异构体。
