Pimenov A M, Uzhevko S A, Toguzov R T
Department of Biochemistry, Central Research Laboratory, N.I. Pirogov 2nd Moscow Medical Institute, U.S.S.R.
J Chromatogr. 1990 Nov 9;520:419-23. doi: 10.1016/0021-9673(90)85128-i.
Isocratic methods are described for the separation of purine compounds in specimens of jejunal mucosa available by peroral biopsy from patients with coeliac disease. The first mode provided separation of both the ionic nucleoside monophosphates and the non-ionic nitrogen bases and nucleosides. The second mode was applicable to analyses of the nucleoside mono-, di- and triphosphates only. These chromatographic procedures were carried out with tetrabutylammonium phosphate as an ion-pair modifier and different concentrations of acetonitrile in the mobile phase. The modes are flexible in that they permit the optimization of the separation of these compounds according to the purpose of the investigation. The results of studies on purine metabolite patterns in coeliac mucosa are discussed.
描述了等度洗脱法用于分离乳糜泻患者经口活检获得的空肠黏膜标本中的嘌呤化合物。第一种模式可分离离子型核苷单磷酸以及非离子型氮碱基和核苷。第二种模式仅适用于核苷单磷酸、二磷酸和三磷酸的分析。这些色谱程序以磷酸四丁铵作为离子对改性剂，并在流动相中使用不同浓度的乙腈进行。这些模式具有灵活性，因为它们允许根据研究目的优化这些化合物的分离。讨论了乳糜泻黏膜中嘌呤代谢物模式的研究结果。
