Fürst W, Hallström S
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
J Chromatogr. 1992 Jul 1;578(1):39-44. doi: 10.1016/0378-4347(92)80222-c.
An ion-pair reversed-phase high-performance liquid chromatographic method is described for the separation and quantification of myocardial nucleotides, nucleosides, their metabolites and creatine phosphate-related compounds in a single run. Separation of a standard mixture containing 21 compounds was achieved on a 5-microns Hypersil ODS column with a 5-min isocratic elution (buffer: 0.1 M NaH2PO4, pH 5.5, containing 5.9 mM tetrabutylammonium hydrogen-sulphate) followed by a slow linear gradient to 17% acetonitrile. The method was applied to extracts of freeze-clamped rat heart tissue samples as well as to extracts of neonatal rat heart cardiomyocytes, and it provided good resolution of high-energy phosphates, including creatine phosphate, as well as of their degradation products.
本文描述了一种离子对反相高效液相色谱法，可在一次运行中分离和定量心肌中的核苷酸、核苷、它们的代谢产物以及磷酸肌酸相关化合物。在一根5微米的Hypersil ODS柱上，通过5分钟的等度洗脱（缓冲液：0.1 M磷酸二氢钠，pH 5.5，含5.9 mM硫酸氢四丁铵），然后缓慢线性梯度至17%乙腈，实现了对含21种化合物的标准混合物的分离。该方法应用于冷冻钳夹的大鼠心脏组织样本提取物以及新生大鼠心脏心肌细胞提取物，它对高能磷酸盐（包括磷酸肌酸）及其降解产物提供了良好的分离度。
