Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
Int J Food Sci Nutr. 2011 Feb;62(1):20-5. doi: 10.3109/09637486.2010.511164. Epub 2010 Sep 27.
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels declined while vitamin A levels were maintained. α-Carotenes and β-carotenes were not detected in the feces of hypercarotenemics but were present in non-hypercarotenemics.
高胡萝卜血症通过皮肤发黄来诊断。本研究旨在研究报告中高胡萝卜血症患者的类胡萝卜素、其代谢物和维生素 A 水平，以及停止摄入富含类胡萝卜素的食物后血清类胡萝卜素的变化，并确定高胡萝卜血症和非高胡萝卜血症患者的粪便中的类胡萝卜素。在 2 至 3 个月的时间内，对 35 名高胡萝卜血症患者进行了检测。从 5 名高胡萝卜血症患者和 8 名非高胡萝卜血症患者的粪便中提取并进行反相高效液相色谱分析。向这些高胡萝卜血症儿童的父母（n=35）发放了一份调查问卷。血清 α-和 β-胡萝卜素分别为 119μg/dl 至痕量和 149μg/dl 至痕量，单羟基代谢物分别为 214μg/dl 至不可检测和多羟基代谢物为 823μg/dl 至 7.0μg/dl。纵向研究表明，血清类胡萝卜素水平下降，而维生素 A 水平保持不变。高胡萝卜血症患者的粪便中未检测到α-胡萝卜素和β-胡萝卜素，但在非高胡萝卜血症患者中存在。
