Department of Oral Medicine, College of Medicine, Second Affiliated Hospital, Zhejiang University, 88, Jiefang Road, Hang zhou, China.
Arch Oral Biol. 2010 Dec;55(12):988-94. doi: 10.1016/j.archoralbio.2010.08.007.
To test the hypothesis that experimental occlusal interferences increase the nerve growth factor (NGF) levels in periodontal tissues and cause an up-regulation of preprotachykinin-A (PPTA) mRNA.
NGF is related to hyperalgesia and inflammation. PPTA mRNA, a primer of substance P, is a possible factor in the aetiology of pain.
Experimental interferences were created by placing inlays in the right maxillary molars of 15 dogs. The right side molars formed the experimental group. The left side molars served as controls. Three dogs with cavities prepared without changing the occlusion formed a sham group. The dogs in the first group were sacrificed, 3 at each time, after 3, 7, 14, 30, and 60 days. The sham group was sacrificed after 14 days. The levels of NGF in periodontal tissues and PPTA mRNA in the trigeminal ganglions were detected by ELISA and TR-PCR. Comparisons were made with paired t-tests and a multivariate MANOVA test.
On all measurement days, there were higher levels of NGF mRNA, PPTA mRNA, and NGF on the experimental than on the control side in 14 of 15 comparisons and in the sham group. NGF production in periodontium was time-dependent. No differences in NGF protein levels were observed between the control and the sham groups.
The results which need confirmation in further tests are of clinical interest. They indicate that occlusal experimental interferences may be an etiologic factor in oral facial pain by increasing mRNA and NGF protein levels in the periodontal tissues.
验证实验性咬合干扰是否会增加牙周组织中的神经生长因子（NGF）水平，并导致前速激肽原-A（PPTA）mRNA 的上调。
NGF 与痛觉过敏和炎症有关。PPTA mRNA 是 P 物质的前体，可能是疼痛病因的一个因素。
通过在上颌磨牙的右侧放置嵌体来产生实验性干扰。右侧磨牙为实验组。左侧磨牙为对照组。在不改变咬合的情况下，对 3 只狗进行了龋齿制备，形成了假手术组。第一组的狗在 3、7、14、30 和 60 天后，每次 3 只被处死。假手术组在 14 天后处死。通过 ELISA 和 TR-PCR 检测牙周组织中 NGF 水平和三叉神经节中 PPTA mRNA。通过配对 t 检验和多元 MANOVA 检验进行比较。
在所有测量日，在 15 次比较中的 14 次和假手术组中，实验组的 NGF mRNA、PPTA mRNA 和 NGF 水平均高于对照组。牙周组织中的 NGF 产生具有时间依赖性。在对照组和假手术组之间未观察到 NGF 蛋白水平的差异。
这些结果具有临床意义，需要进一步的测试来证实。它们表明，咬合实验性干扰可能通过增加牙周组织中的 mRNA 和 NGF 蛋白水平成为口腔颌面疼痛的病因。
