Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Phytomedicine. 2011 Mar 15;18(5):402-7. doi: 10.1016/j.phymed.2010.08.011.
Mitragyna speciosa Korth. leaves have been used for decades as a traditional medicine to treat diarrhea, diabetes and to improve blood circulation by natives of Malaysia, Thailand and other regions of Southeast Asia. Mitragynine is the major active alkaloid in the plant. To date, the role of mitragynine in psychological disorders such as depression is not scientifically evaluated. Hence, the present investigation evaluates the antidepressant effect of mitragynine in the mouse forced swim test (FST) and tail suspension test (TST), two models predictive of antidepressant activity and the effect of mitragynine towards neuroendocrine system of hypothalamic-pituitary-adrenal (HPA) axis by measuring the corticosterone concentration of mice exposed to FST and TST. An open-field test (OFT) was used to detect any association of immobility in the FST and TST with changes in motor activity of mice treated with mitragynine. In the present study, mitragynine at dose of 10 mg/kg and 30 mg/kg i.p. injected significantly reduced the immobility time of mice in both FST and TST without any significant effect on locomotor activity in OFT. Moreover, mitragynine significantly reduced the released of corticosterone in mice exposed to FST and TST at dose of 10 mg/kg and 30 mg/kg. Overall, the present study clearly demonstrated that mitragynine exerts an antidepressant effect in animal behavioral model of depression (FST and TST) and the effect appears to be mediated by an interaction with neuroendocrine HPA axis systems.
Korth 的马缨丹属植物叶子被马来西亚、泰国和东南亚其他地区的土著居民使用了几十年，用于治疗腹泻、糖尿病，并改善血液循环。帽柱木碱是该植物中的主要活性生物碱。迄今为止，帽柱木碱在抑郁症等心理障碍中的作用尚未得到科学评估。因此，本研究评估了帽柱木碱在小鼠强迫游泳试验（FST）和悬尾试验（TST）中的抗抑郁作用，这两种模型可预测抗抑郁活性，以及帽柱木碱对下丘脑-垂体-肾上腺（HPA）轴神经内分泌系统的影响通过测量暴露于 FST 和 TST 的小鼠的皮质酮浓度。旷场试验（OFT）用于检测 FST 和 TST 中不动性与用帽柱木碱治疗的小鼠运动活动变化之间的任何关联。在本研究中，10 mg/kg 和 30 mg/kg i.p. 注射的帽柱木碱显著减少了 FST 和 TST 中小鼠的不动时间，而对 OFT 中的运动活性没有任何显著影响。此外，帽柱木碱在 10 mg/kg 和 30 mg/kg 剂量下显著降低了暴露于 FST 和 TST 的小鼠的皮质酮释放。总的来说，本研究清楚地表明，帽柱木碱在抑郁动物行为模型（FST 和 TST）中表现出抗抑郁作用，其作用似乎是通过与神经内分泌 HPA 轴系统相互作用介导的。
