Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Neuron. 2010 Sep 23;67(6):1048-61. doi: 10.1016/j.neuron.2010.08.026.
Classical studies have related the spiking of selected neocortical neurons to behavior, but little is known about activity sampled from the entire neural population. We recorded from neurons selected independent of spiking, using cell-attached recordings and two-photon calcium imaging, in the barrel cortex of mice performing an object localization task. Spike rates varied across neurons, from silence to >60 Hz. Responses were diverse, with some neurons showing large increases in spike rate when whiskers contacted the object. Nearly half the neurons discriminated object location; a small fraction of neurons discriminated perfectly. More active neurons were more discriminative. Layer (L) 4 and L5 contained the highest fractions of discriminating neurons (∼63% and 79%, respectively), but a few L2/3 neurons were also highly discriminating. Approximately 13,000 spikes per activated barrel column were available to mice for decision making. Coding of object location in the barrel cortex is therefore highly redundant.
经典研究将选定的新皮层神经元的尖峰活动与行为联系起来，但对于从整个神经元群体中采样的活动知之甚少。我们在执行物体定位任务的小鼠的桶状皮层中，使用细胞贴附记录和双光子钙成像，对独立于尖峰活动选择的神经元进行记录。神经元的尖峰率差异很大，从沉默到>60 Hz。反应多种多样，有些神经元在触须接触物体时尖峰率大幅增加。近一半的神经元能够区分物体的位置；一小部分神经元能够完美区分。更活跃的神经元具有更高的辨别能力。第 4 层（L4）和第 5 层（L5）包含的辨别神经元比例最高（分别约为 63%和 79%），但少数 L2/3 神经元也具有很高的辨别能力。大约 13000 个激活的桶状柱的尖峰可供小鼠进行决策。因此，桶状皮层中物体位置的编码具有高度的冗余性。
