Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Phutthamonthon 4 Road, Nakhon Pathom 73170, Thailand.
Virus Res. 2011 Jan;155(1):131-6. doi: 10.1016/j.virusres.2010.09.009. Epub 2010 Sep 24.
Penaeus stylirostris densovirus (PstDNV) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei. Although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded RNA (dsRNA) or siRNA has given promising results. In present study, dsRNAs corresponding to non-structural (ORF1 and ORF2 overlapping sequence) and structural (ORF3) genes of PstDNV were investigated for their potency to inhibit PstDNV replication in the shrimp. Periodically injection of either ORF1-2 dsRNA or ORF3 dsRNA at three days interval into L. vannamei resulted in substantial inhibition of PstDNV infection. In addition, a possibility for a therapeutic application of dsRNA in PstDNV-infected shrimp was demonstrated by the efficient suppression of PstDNV replication in L. vannamei when the ORF1-2 dsRNA was delivered into the shrimp within 24h post-PstDNV injection. Hence, our results established both the preventive and therapeutic potency of dsRNA to inhibit PstDNV in L. vannamei that could be applied as a potential treatment of PstDNV infection in shrimp.
刀额新对虾浓核病毒（Penaeus stylirostris densovirus，PstDNV）感染广泛存在于对虾中，特别是在经济上重要的种类，如黑虎虾 Penaeus monodon 和凡纳滨对虾 Litopenaeus vannamei。虽然虾类病毒性疾病的有效预防方法尚未得到很好的确立，但针对病毒特异性双链 RNA（dsRNA）或 siRNA 的治疗已取得了有希望的结果。在本研究中，研究了与 PstDNV 的非结构（ORF1 和 ORF2 重叠序列）和结构（ORF3）基因相对应的 dsRNA 抑制虾中 PstDNV 复制的能力。定期每隔三天向凡纳滨对虾注射 ORF1-2 dsRNA 或 ORF3 dsRNA，可显著抑制 PstDNV 感染。此外，当在注射 PstDNV 后 24 小时内将 ORF1-2 dsRNA 递送至虾中时，dsRNA 对 PstDNV 复制的有效抑制证明了 dsRNA 在 PstDNV 感染的虾中具有治疗应用的可能性。因此，我们的结果确立了 dsRNA 在凡纳滨对虾中抑制 PstDNV 的预防和治疗效力，可作为虾类 PstDNV 感染的潜在治疗方法。
