[Phenotyping of apolipoprotein E: immunoblotting after isoelectric focusing in immobilized pH gradients].

Apolipoprotein E is a major ligand for cellular lipoprotein receptors and plays an important role in the catabolism of triglyceride-rich lipoproteins. The polymorphism of apo E is associated with familial dysbetalipoproteinemia, LDL-cholesterol, and cardiovascular risk. In this report a method for the analysis of apo E isoforms in immobilized pH gradients is presented that does not require isolation apo E containing lipoproteins by ultracentrifugation.