März W, Cezanne S, Gross W
Gustav Embden-Zentrum der Biologischen Chemie, J.W. Goethe-Universität, Frankfurt am Main.
Klin Wochenschr. 1990;68 Suppl 22:113-4.
Apolipoprotein E is a major ligand for cellular lipoprotein receptors and plays an important role in the catabolism of triglyceride-rich lipoproteins. The polymorphism of apo E is associated with familial dysbetalipoproteinemia, LDL-cholesterol, and cardiovascular risk. In this report a method for the analysis of apo E isoforms in immobilized pH gradients is presented that does not require isolation apo E containing lipoproteins by ultracentrifugation.
载脂蛋白E是细胞脂蛋白受体的主要配体，在富含甘油三酯的脂蛋白的分解代谢中起重要作用。载脂蛋白E的多态性与家族性异常β脂蛋白血症、低密度脂蛋白胆固醇和心血管风险相关。在本报告中，提出了一种在固定化pH梯度中分析载脂蛋白E异构体的方法，该方法不需要通过超速离心分离含载脂蛋白E的脂蛋白。
