遗传性高胆固醇血症大鼠（RICO）中载脂蛋白E的表征与定量分析。

Characterization and quantification of apolipoprotein E in the genetically hypercholesterolemic rat (RICO).

作者信息

Cardona-Sanclemente L E, Sultan F, Griglio S

机构信息

Pathopharmacology Unit, William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London, England.

出版信息

Rev Esp Fisiol. 1996 Mar;52(1):15-22.

PMID:8774081

Abstract

The levels of apolipoprotein E (apo E) in serum and in isolated lipoprotein fractions from genetically hypercholesterolemic rats (RICO) and their normocholesterolemic controls (SW), were evaluated by immunoblotting, and their polymorphism was analyzed by isoelectrofocusing. The present results confirm previous findings from a qualitative SDS-PAGE analysis. When measured by immunoblotting, total apo E increased by 35% in the RICO rat plasma. In the different lipoprotein fractions, apo E in RICO rat increased in low density lipoproteins; LDL1, LDL2 (apo E rich HDL1) and high density lipoproteins (HDL2, HDL3). Only in the chylomicron fraction did apo E decrease. The two-dimensional electrophoretic method demonstrated that four isoproteins designated E-1, E-2, E-3 and E-4 are present at a pI range from 5.36 to 5.56 in RICO rat as well as in control SW rat.

摘要

通过免疫印迹法评估了遗传性高胆固醇血症大鼠（RICO）及其正常胆固醇血症对照大鼠（SW）血清和分离的脂蛋白组分中载脂蛋白E（apo E）的水平，并通过等电聚焦分析了其多态性。目前的结果证实了先前定性SDS-PAGE分析的结果。通过免疫印迹法测量时，RICO大鼠血浆中总apo E增加了35%。在不同的脂蛋白组分中，RICO大鼠的apo E在低密度脂蛋白（LDL1、LDL2（富含apo E的HDL1））和高密度脂蛋白（HDL2、HDL3）中增加。仅在乳糜微粒组分中apo E减少。二维电泳法表明，在RICO大鼠以及对照SW大鼠中，四种异蛋白E-1、E-2、E-3和E-4存在于pI范围为5.36至5.56之间。