Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
J Anim Sci. 2011 Jan;89(1):113-23. doi: 10.2527/jas.2010-3127. Epub 2010 Sep 24.
Nutrient digestibility in distillers dried grains with solubles (DDGS) is limited by physical constraints such as particle size and by biochemical limitations such as phytate and fiber or nonstarch polysaccharides (NSP). To determine the separate effects of these limitations on nutrient digestibility, ground DDGS (383 µm) supplemented with phytase (0 or 250 units/kg of feed) and xylanase (0 or 4,000 units/kg of feed) was evaluated in a 2 × 2 factorial arrangement of treatments together with unground DDGS (517 µm) and an N-free diet in a 6 × 6 Latin square. Cofermented wheat and corn DDGS contained 8.6% moisture, 31.0% CP, 1.04% Lys, 8.0% ether extract, 2.0% starch, 40% NDF, and 0.85% P (as-is basis). Diets contained 43.7% DDGS as the sole source of AA; the digesta from pigs fed the N-free diet served to subtract basal endogenous AA losses and as control for energy digestibility. Six ileal-cannulated barrows (37.1 ± 0.8 kg of BW) were fed 6 diets at 2.8 × maintenance for DE in six 9-d periods. Feces and ileal digesta were collected for 2 d each. The apparent ileal digestibility (AID) of GE and apparent total tract digestibility (ATTD) of GE and NDF were 2.3, 0.5, and 5.1%-units greater (P < 0.05) for the ground than unground DDGS diet, respectively. Consequently, the ATTD of GE was 1.3%-units greater (P < 0.05) and the DE content was 0.06 Mcal/kg greater (P < 0.05) for ground than unground DDGS, respectively. Grinding of DDGS did not affect (P > 0.05) the ATTD of crude fiber, ADF, P, and Ca in diets. Grinding of DDGS increased (P < 0.05) the AID of most AA in diets including Lys, Met, and Thr by 6.9, 1.1, and 1.7%-units, respectively. Grinding of DDGS increased (P < 0.05) the SID of Lys by 6.2%-units and SID content of Lys and Thr by 0.06 and 0.02%-units, respectively. Phytase and xylanase did not interact (P > 0.05) to affect nutrient digestibility. Phytase increased (P < 0.001) the ATTD of P by 10.5%-units, but did not affect (P > 0.05) AA digestibility. Xylanase did not affect nutrient digestibility. In conclusion, particle size is an important physical characteristic affecting digestibility of energy and AA, but not P in DDGS. Phytate in DDGS limits digestibility of P, but not energy and AA. The substrate for xylanase in DDGS did not hinder energy and AA digestibility.
在含有可溶物的干酒糟(DDGS)中,养分消化率受到物理限制,如颗粒大小,以及生化限制,如植酸和纤维或非淀粉多糖(NSP)。为了确定这些限制对养分消化率的单独影响,评估了在含有植酸酶(0 或 250 单位/千克饲料)和木聚糖酶(0 或 4000 单位/千克饲料)的磨碎 DDGS(383 µm)和未磨碎 DDGS(517 µm)以及无氮饮食的 2×2 因子处理组合,以及无氮饮食在 6×6 拉丁方中的应用。共发酵的小麦和玉米 DDGS 含有 8.6%的水分、31.0%的 CP、1.04%的 Lys、8.0%的乙醚提取物、2.0%的淀粉、40%的 NDF 和 0.85%的 P(原样基础)。日粮中含有 43.7%的 DDGS 作为唯一的 AA 来源;饲喂无氮日粮的猪的回肠食糜用于减去基础内源性 AA 损失,并作为能量消化率的对照。6 头回肠插管的阉公猪(37.1±0.8kg BW)在 6 个 9 天的试验中以 2.8×维持需要的 DE 水平喂食 6 种日粮。收集 2 天的粪便和回肠食糜。与未磨碎 DDGS 日粮相比,磨碎 DDGS 日粮的 GE 表观回肠消化率(AID)和 GE 和 NDF 的总肠道表观消化率(ATTD)分别提高了 2.3、0.5 和 5.1%(P<0.05)。因此,磨碎 DDGS 日粮的 GE ATTD 提高了 1.3%(P<0.05),DE 含量提高了 0.06 Mcal/kg(P<0.05)。磨碎 DDGS 对日粮中粗纤维、ADF、P 和 Ca 的 ATTD 没有影响(P>0.05)。磨碎 DDGS 增加了日粮中大多数 AA 的 AID,包括 Lys、Met 和 Thr,分别提高了 6.9、1.1 和 1.7%(P<0.05)。磨碎 DDGS 增加了 Lys 的SID 6.2%,以及 Lys 和 Thr 的 SID 含量分别增加了 0.06 和 0.02%(P<0.05)。植酸酶和木聚糖酶没有相互作用(P>0.05)来影响养分消化率。植酸酶提高了 P 的 ATTD 10.5%(P<0.001),但不影响 AA 消化率(P>0.05)。木聚糖酶不影响养分消化率。总之,颗粒大小是影响 DDGS 中能量和 AA 消化率而不是 P 消化率的重要物理特性。DDGS 中的植酸限制了 P 的消化率,但不影响能量和 AA。DDGS 中木聚糖酶的底物并不妨碍能量和 AA 的消化率。