Passos Adsos Adami, Moita Vitor Hugo Cardoso, Kim Sung Woo
Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
Anim Biosci. 2023 Dec;36(12):1869-1879. doi: 10.5713/ab.23.0212. Epub 2023 Aug 28.
This study was to evaluate the effects of individual or combinational use of phytase, protease, and xylanase on total tract digestibility of corn, soybean meal, and distillers dried grains with soluble (DDGS) fed to pigs.
Each experiment had four 4×4 Latin squares using 16 barrows. Each period had 5-d adaptation and 3-d collection. All experiments had: CON (no enzyme); Phy (CON+phytase); Xyl (CON+xylanase); Pro (CON+protease); Phy+Xyl; Phy+Pro, Xyl+Pro, Phy+Xyl+Pro. Each Latin square had 'CON, Phy, Xyl, and Phy+Xyl'; 'CON, Phy, Pro, and Phy+Pro'; 'CON, Pro, Xyl, and Xyl+Pro'; and 'Phy+Xyl, Phy+Pro, Xyl+Pro, Phy+Xyl+Pro'.
The digestible energy (DE), metabolizable energy (ME), and nitrogen retention (NR) of corn were not affected by enzymes but the apparent total tract digestibility (ATTD) of phosphorus (P) was improved (p<0.01) by Phy. The DE and ATTD dry matter (DM) in soybean meal were increased (p<0.05) by Phy+Pro and the ATTD P was improved (p<0.01) by Phy, Phy+Pro, and Phy+Xyl. The DE, ME, and ATTD DM in DDGS were improved (p<0.05) by Phy+Xyl and the ATTD P was improved (p<0.01) by Phy, Phy+Pro, and Phy+Xyl.
Phytase individually or in combination with xylanase and protease improved the Ca and P digestibility of corn, soybean meal, and DDGS, from the hydrolysis of phytic acid. The supplementation of protease was more effective when combined with phytase and xylanase in the soybean meal and DDGS possibly due to a higher protein content in these feedstuffs. Xylanase was more effective in DDGS diets due to the elevated levels of non-starch polysaccharides in these feedstuffs. However, when xylanase was combined with phytase, it demonstrated a higher efficacy improving the nutrient digestibility of pigs. Overall, combinational uses of feed enzymes can be more efficient for nutrient utilization in soybean meal and DDGS than single enzymes.
本研究旨在评估单独或组合使用植酸酶、蛋白酶和木聚糖酶对饲喂猪的玉米、豆粕和玉米酒糟可溶性物(DDGS)全肠道消化率的影响。
每个实验采用4×4拉丁方设计,使用16头公猪。每个阶段有5天的适应期和3天的收集期。所有实验处理包括:对照组(不添加酶);植酸酶组(对照组+植酸酶);木聚糖酶组(对照组+木聚糖酶);蛋白酶组(对照组+蛋白酶);植酸酶+木聚糖酶组;植酸酶+蛋白酶组;木聚糖酶+蛋白酶组;植酸酶+木聚糖酶+蛋白酶组。每个拉丁方包含“对照组、植酸酶组、木聚糖酶组和植酸酶+木聚糖酶组”;“对照组、植酸酶组、蛋白酶组和植酸酶+蛋白酶组”;“对照组、蛋白酶组、木聚糖酶组和木聚糖酶+蛋白酶组”;以及“植酸酶+木聚糖酶组、植酸酶+蛋白酶组、木聚糖酶+蛋白酶组、植酸酶+木聚糖酶+蛋白酶组”。
玉米的消化能(DE)、代谢能(ME)和氮保留(NR)不受酶的影响,但植酸酶可提高磷(P)的表观全肠道消化率(ATTD)(p<0.01)。植酸酶+蛋白酶可提高豆粕中DE和干物质(DM)的ATTD(p<0.05),植酸酶、植酸酶+蛋白酶和植酸酶+木聚糖酶可提高豆粕中P的ATTD(p<0.01)。植酸酶+木聚糖酶可提高DDGS中DE、ME和DM的ATTD(p<0.05),植酸酶、植酸酶+蛋白酶和植酸酶+木聚糖酶可提高DDGS中P的ATTD(p<0.01)。
单独或与木聚糖酶和蛋白酶组合使用植酸酶,通过植酸水解提高了玉米、豆粕和DDGS中钙和磷的消化率。在豆粕和DDGS中,蛋白酶与植酸酶和木聚糖酶组合时更有效,可能是因为这些饲料中蛋白质含量较高。由于这些饲料中非淀粉多糖含量较高,木聚糖酶在DDGS日粮中更有效。然而,当木聚糖酶与植酸酶组合时,在提高猪的养分消化率方面表现出更高的功效。总体而言,饲料酶的组合使用在豆粕和DDGS的养分利用方面比单一酶更有效。