[银屑病患者受累皮肤活检中诱导型一氧化氮合酶和低氧诱导因子-1α的表达与血管生成]

[Expression of iNOS and HIF-1α with angiogenesis in affected skin biopsies from patients with psoriasis].

作者信息

Li Yongjian, Zhang Guiying, Xiao Rong, Chen Huan, Wen Haiquan

机构信息

Department of Dermatology, The First Affiliated Hospital of Nanhua University, Hengyang Hunan, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Sep;35(9):952-7. doi: 10.3969/j.issn.1672-7347.2010.09.009.

DOI:10.3969/j.issn.1672-7347.2010.09.009

PMID:20871160

Abstract

OBJECTIVE

To investigate the expression of inducible nitric oxide synthase (iNOS) and hypoxia-inducible factor 1(HIF-1) α in psoriatic lesions,and to explore their relationship with angiogenesis in psoriasis.

METHODS

HIF-1α and iNOS protein were detected by immunohistochemistry and Western blot in 32 cases of psoriasis and 20 healthy controls,and CD34 marking vascular endothelial cells were used to measure the microvascular density (MVD).

RESULTS

The expressions of HIF-1α and iNOS protein were very weak in the control skin, but very strong in psoriatic lesions, which showed significant difference in the expressions of iNOS and HIF-1α between the psoriasis and the control group(P<0.05). Expression of HIF-1α (r=0.743, P<0.01) and iNOS (r=0.639,P<0.01) had positive correlation with MVD in psoriasis respectively. There was a positive correlation between iNOS and HIF-1α expression in psoriasis (r=0.717, P<0.01).

CONCLUSION

Both iNOS and HIF-1α have high expression in psoriasis and might play an important role in the genesis and development of psoriasis.

摘要

目的

探讨诱导型一氧化氮合酶（iNOS）和缺氧诱导因子1（HIF-1）α在银屑病皮损中的表达情况，并探讨它们与银屑病血管生成的关系。

方法

采用免疫组织化学和蛋白质印迹法检测32例银屑病患者及20例健康对照者皮肤组织中HIF-1α和iNOS蛋白的表达，并用CD34标记血管内皮细胞，检测微血管密度（MVD）。

结果

正常对照皮肤组织中HIF-1α和iNOS蛋白表达较弱，而银屑病皮损组织中表达较强，银屑病组与对照组iNOS和HIF-1α的表达差异有统计学意义（P<0.05）。银屑病组织中HIF-1α（r=0.743，P<0.01）和iNOS（r=0.639，P<0.01）的表达分别与MVD呈正相关。银屑病组织中iNOS与HIF-1α的表达呈正相关（r=0.717，P<0.01）。