Liu Yang, Cui Yong-Hua, Yu Lin-Lin, Liu Zheng, Zhang Ping
Department of Otorhinolaryngology Head and Neck Surgery, Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2005 May;40(5):366-70.
To detect the expression and distribution of hypoxia-inducible factor-1alpha (HIF-1alpha) in nasal polyps, to explore the relationship of HIF-1alpha with vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS), and to investigate the role of HIF-1alpha in the pathogenetic mechanism of nasal polyps.
Using the techniques of immunohistochemistry and reverse transcription polymerase chain reaction, the expressions of HIF-1alpha, VEGF, iNOS protein and HIF-1alpha mRNA were examined. The specimens were obtained from patients underwent SMR surgery during the same period, including thirty-one nasal polyps and ten inferior turbinate tissues.
According to immunohistochemical research, the expressions of HIF-1alpha, VEGF and iNOS were higher in nasal polyps than those in inferior turbinate tissue (P < 0.01); The result of RT-PCR showed that there was no significant difference between the expression of HIF-1alpha mRNA in nasal polyps and inferior turbinate tissues (P > 0.05); No correlation was found between the expression of HIF-1alpha and the clinical stages of nasal polyps (F = 0.42, P > 0.05); There was a positive correlation between the expression of HIF-1alpha and that of VEGF (r = 0.630, P < 0.05). No significant correlation was found between the expression of HIF-1alpha and that of Inos (r = 0.144, P > 0.05).
Although the mRNA level of HIF-1alpha did not change much, the expression of its protein was significantly higher in nasal polyps than in inferior turbinate tissues. There was no correlation between the expression of HIF-1alpha and the clinical stages. A positive correlation was found between HIF-1alpha and VEGF, but no correlation was detected between the HIF-1alpha and iNOS. In conclusion, our study indicates that hypoxia may play an important role in the pathogenetic mechanism of nasal polyps.
检测低氧诱导因子-1α（HIF-1α）在鼻息肉中的表达及分布，探讨HIF-1α与血管内皮生长因子（VEGF）和诱导型一氧化氮合酶（iNOS）的关系，以及HIF-1α在鼻息肉发病机制中的作用。
采用免疫组织化学和逆转录聚合酶链反应技术，检测HIF-1α、VEGF、iNOS蛋白及HIF-1α mRNA的表达。标本取自同期行鼻窦内窥镜手术的患者，包括31例鼻息肉组织和10例下鼻甲组织。
免疫组织化学研究显示，鼻息肉中HIF-1α、VEGF和iNOS的表达高于下鼻甲组织（P < 0.01）；逆转录聚合酶链反应结果显示，鼻息肉组织与下鼻甲组织中HIF-1α mRNA表达差异无统计学意义（P > 0.05）；HIF-1α的表达与鼻息肉临床分期无关（F = 0.42，P > 0.05）；HIF-1α的表达与VEGF的表达呈正相关（r = 0.630，P < 0.05）。HIF-1α的表达与iNOS的表达无显著相关性（r = 0.144，P > 0.05）。
虽然HIF-1α的mRNA水平变化不大，但其蛋白在鼻息肉中的表达明显高于下鼻甲组织。HIF-1α的表达与临床分期无关。HIF-1α与VEGF呈正相关，但与iNOS无相关性。总之，本研究表明低氧在鼻息肉发病机制中可能起重要作用。
