Mechanical Engineering, Middle East Technical University, TRNC Mersin, Turkey. [corrected]
Electrophoresis. 2010 Sep;31(18):3035-43. doi: 10.1002/elps.201000107.
A novel alternating current-dielectrophoresis microfluidic chip was developed to separate particles and cells continuously by their electric properties. The flow is induced by pressure gradient. A pair of simple, 3-D electrodes was used to achieve a localized nonuniform electric field. Dielectrophoretic force is generated in the transverse direction to the flow by inserting the electrodes along the channel side walls. The localized electric field is important to reduce the Joule heating and any adverse effects of electrical field on biological cells. Latex particles of different sizes and white blood cells (8-12 μm) were manipulated successfully and the separation of 10 μm latex particles and white blood cells based on their different electrical properties was demonstrated.
一种新型的交流电介电泳微流控芯片通过其电学特性连续分离粒子和细胞。流动是由压力梯度引起的。一对简单的三维电极用于实现局部非均匀电场。通过沿通道侧壁插入电极，在横向于流动的方向上产生介电泳力。局部电场对于减少焦耳热和电场对生物细胞的任何不利影响很重要。成功地操纵了不同大小的乳胶粒子和白细胞（8-12μm），并基于它们不同的电学特性展示了对 10μm 乳胶粒子和白细胞的分离。
