Chulkin A M, Vavilova E A, Benevolenskiĭ S V
Mol Biol (Mosk). 2010 Jul-Aug;44(4):677-87.
Penicillium canescens strain F178 is a natural producer of beta-galactosidase and endo-1,4-beta-xylanase. Tanscription of genes bgaS and xylA coding for these proteins is subject to carbon catabolite repression which proceed mainly in filamentous fungi by transcriptional repressor CreA. creA gene of P. canescens was cloned. It was demonstrated that creA transcription is also subject to carbon catabolite repression. CreA protein remains intranuclear independently of nature of carbon source and glucose concentration in culture medium. In vitro experiments confirm availability of four CreA-binding sites in bgaS promoter, four sites in xylA promoter and one such site in creA promoter.
灰绿青霉菌株F178是β-半乳糖苷酶和内切-1,4-β-木聚糖酶的天然生产者。编码这些蛋白质的bgaS和xylA基因的转录受到碳分解代谢物阻遏，这在丝状真菌中主要通过转录阻遏物CreA进行。克隆了灰绿青霉的creA基因。结果表明，creA转录也受到碳分解代谢物阻遏。CreA蛋白在细胞核内保持独立，与培养基中碳源的性质和葡萄糖浓度无关。体外实验证实，bgaS启动子中有四个CreA结合位点，xylA启动子中有四个位点，creA启动子中有一个这样的位点。
