Chulkin A M, Vavilova E A, Benevolenskiĭ S V
Mol Biol (Mosk). 2011 Sep-Oct;45(5):871-8.
Penicillium canescens strain F178 is a natural producer of beta-galactosidase and endo-1,4-beta-xylanase. The transcription of genes bgaS and xylA, coding for these proteins, is subject to carbon catabolite repression. The system for selective isolation of regulatory mutants in P. canescens is developed. Two strains from the mutant collection are studied in details. It is shown that both mutations can be complement by creA gene of P. canescens, encoding global regulator of carbon catabolite repression in filamentous fungi. creA(-) alleles contain frameshift mutations in C-domain of CreA. Gene xylA is derepressed in mutants at transcription level in the presence of D-glucose. A transcription of creA gene in mutants is also derepressed proving effect of autoregulation for this gene.
渐灰青霉F178菌株是β-半乳糖苷酶和内切-1,4-β-木聚糖酶的天然生产者。编码这些蛋白质的bgaS和xylA基因的转录受碳分解代谢物阻遏作用的影响。已开发出用于在渐灰青霉中选择性分离调控突变体的系统。对突变体库中的两个菌株进行了详细研究。结果表明，这两种突变都可以被渐灰青霉的creA基因互补，该基因编码丝状真菌中碳分解代谢物阻遏作用的全局调节因子。creA(-)等位基因在CreA的C结构域中含有移码突变。在存在D-葡萄糖的情况下，xylA基因在突变体的转录水平上解除了阻遏。突变体中creA基因的转录也被解除阻遏，证明了该基因的自动调节作用。
