[白细胞介素-23在变应性鼻炎患者鼻黏膜中的表达及其意义]

[The expression of IL-23 in nasal mucosa of allergic rhinitis patients and its significance].

作者信息

Liu Yang, Liu Zheng, Lu Xiang, Gao Qixue, Cui Yonghua

机构信息

Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Jul;24(14):638-40.

PMID:20873540

Abstract

OBJECTIVE

To investigate the expression of interleukin (IL)-23 in the nasal mucosa of allergic rhinitis patients and its significance.

METHOD

mRNA and protein expression of IL-23 in inferior turbinate mucosa from 12 allergic rhinitis patients and 11 control patients was measured by means of real-time RT-PCR and immunohistochemistry, respectively.

RESULT

IL-23p19 mRNA relative expression level in nasal mucosa was significantly increased in allergic rhinitis patients compared with normal controls (P < 0.01). Immunohistochemical staining demonstrated that IL-23 protein was mainly expressed by infiltrating inflammatory cells in lamina propria and there was increased number of IL-23 positive cells in allergic rhinitis patients in comparison with normal controls. Correlation analysis showed that the mRNA and protein expression level of IL-23 was significantly positively correlated with the number of the inflammatory cells (r = 0.678 and 0.644, respectively; both P < 0.01) and the degree of subepithelial collagen deposition (r = 0.834 and 0.721, respectively; both P < 0.01). IL-23p19 mRNA relative expression level in nasal mucosa was significantly decreased in allergic rhinitis patients who used glucocorticoids compared with controls (P < 0.01).

CONCLUSION

IL-23 may contribute to the chronic inflammation and airway remodelling in allergic rhinitis.

摘要

目的

探讨白细胞介素（IL）-23在变应性鼻炎患者鼻黏膜中的表达及其意义。

方法

分别采用实时逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法检测12例变应性鼻炎患者及11例对照患者下鼻甲黏膜中IL-23的mRNA和蛋白表达。

结果

与正常对照组相比，变应性鼻炎患者鼻黏膜中IL-23p19 mRNA相对表达水平显著升高（P < 0.01）。免疫组织化学染色显示，IL-23蛋白主要由固有层浸润的炎性细胞表达，与正常对照组相比，变应性鼻炎患者中IL-23阳性细胞数量增加。相关性分析表明，IL-23的mRNA和蛋白表达水平与炎性细胞数量（分别为r = 0.678和0.644；均P < 0.01）及上皮下胶原沉积程度（分别为r = 0.834和0.721；均P < 0.01）呈显著正相关。与对照组相比，使用糖皮质激素的变应性鼻炎患者鼻黏膜中IL-23p19 mRNA相对表达水平显著降低（P < 0.01）。