Hu Yan-shan, Zhu Jian-hua, Jiang Bo, Yu Rong-min
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2010 May;33(5):662-5.
To investigate the biotransformation of artemisinic acid by cell suspension cultures of Cephalotaxus fortunei and Artemisia annua.
Artemisinic acid was added into to the media of the suspension cells of Cephalotaxus fortunei and Artemisia annua in their logarithmic growth phase. The biotransfromed product was detected with HPLC and isolated by silica gel column, Sephadex LH20 and ODS chromatography methods. The chemical structure of biotransformed product was elucidated on the basis of physical-chemical properties and spectroscopic data. Otherwise, the influence of co-cultured time on conversion ratio was investigated with HPLC.
One biotransformed product, 3-alpha-hydroxyartemisinic acid, was obtained after two days of artemisinic acid administration to the suspension cells of Cephalotaxus fortunei and Artemisia annua. The optimal co-cultured time in suspension cells of Cephalotaxus fortunei was 2 days with the highest biotransformation rate of 8.42%, and in the case of Artemisia annua, it was 3 days and 3.95% respectively.
It was the first time for the biotransformation of artemisinic acid to 3-alpha-hydroxyartemisinic acid by using cell suspension cultures of Cephalotaxus fortunei and Artemisia annua.
研究三尖杉和青蒿细胞悬浮培养物对青蒿酸的生物转化。
在三尖杉和青蒿悬浮细胞对数生长期的培养基中添加青蒿酸。采用高效液相色谱法检测生物转化产物，并用硅胶柱、葡聚糖凝胶LH20和十八烷基硅烷键合硅胶色谱法进行分离。根据物理化学性质和光谱数据阐明生物转化产物的化学结构。此外，采用高效液相色谱法研究共培养时间对转化率的影响。
在三尖杉和青蒿悬浮细胞中添加青蒿酸两天后，得到一种生物转化产物3-α-羟基青蒿酸。三尖杉悬浮细胞的最佳共培养时间为2天，最高生物转化率为8.42%；青蒿悬浮细胞的最佳共培养时间为3天，最高生物转化率为3.95%。
首次利用三尖杉和青蒿细胞悬浮培养物将青蒿酸生物转化为3-α-羟基青蒿酸。
