Tian Na, Li Juan, Liu Shuoqian, Huang Jianan, Li Xun, Liu Zhonghua
Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Natural Products Research Center, College of Horticulture and Hardening, Hunan Agricultural University, Changsha, 410128, China.
Biomed Chromatogr. 2012 Jun;26(6):708-13. doi: 10.1002/bmc.1719. Epub 2011 Sep 19.
It is still a major challenge to simultaneously isolate artemisinin and its precursors, especially dihydroartemisinic acid and artemisinic acid, from herbal Artemisia annua. A rapid, economical and automatical chromatographic separation process to isolate and purify artemisinin, dihydroartemisinic acid and artemisinic acid at the same time on a preparative scale was developed. The procedure included solvent extraction of ground Artemisia annua leaves by refluxing and purification of crude extract by preparative reverse-phase high-performance liquid chromatography (RP-HPLC). Fractions containing artemisinin and its precursors were collected and identified by gas chromatography and mass spectrometry. High purity of artemisinin, dihydroartemisinic acid and artemisinic acid was obtained by preparative HPLC with a C(18) column and 60% acetonitrile in water as the mobile phase. The techniques described here are useful tools for the preparative-scale isolation of artemisinin and its precursors in a fast, cost-effective and environmental friendly manner.
从青蒿中同时分离青蒿素及其前体，特别是二氢青蒿酸和青蒿酸，仍然是一项重大挑战。开发了一种快速、经济且自动化的色谱分离方法，可在制备规模上同时分离和纯化青蒿素、二氢青蒿酸和青蒿酸。该方法包括通过回流对磨碎的青蒿叶进行溶剂萃取，以及通过制备型反相高效液相色谱（RP-HPLC）对粗提物进行纯化。收集含有青蒿素及其前体的馏分，并通过气相色谱和质谱进行鉴定。使用C(18)柱和60%乙腈水溶液作为流动相，通过制备型HPLC获得了高纯度的青蒿素、二氢青蒿酸和青蒿酸。这里描述的技术是以快速、经济高效且环保的方式进行青蒿素及其前体的制备规模分离的有用工具。
