Department of Engineering Sciences, Division of Electron Microscopy and Nanoengineering, The Ångström Laboratory, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.
J Phys Chem B. 2010 Oct 21;114(41):13255-62. doi: 10.1021/jp105756b.
The present work provides the first real-space analysis of nanobead-DNA coil interactions. Immobilization of oligonucleotide-functionalized magnetic nanobeads in rolling circle amplified DNA-coils was studied by complex magnetization measurements and transmission electron microscopy (TEM), and a statistical analysis of the number of beads hybridized to the DNA-coils was performed. The average number of beads per DNA-coil using the results from both methods was found to be around 6 and slightly above 2 for samples with 40 and 130 nm beads, respectively. The TEM analysis supported an earlier hypothesis that 40 nm beads are preferably immobilized in the interior of DNA-coils whereas 130 nm beads, to a larger extent, are immobilized closer to the exterior of the coils. The methodology demonstrated in the present work should open up new possibilities for characterization of interactions of a large variety of functionalized nanoparticles with macromolecules, useful for gaining more fundamental understanding of such interactions as well as for optimizing a number of biosensor applications.
本工作首次对纳米珠-DNA 线圈相互作用进行了实空间分析。通过复磁化测量和透射电子显微镜（TEM）研究了寡核苷酸功能化磁性纳米珠在滚环扩增 DNA 线圈中的固定化，并对与 DNA 线圈杂交的珠的数量进行了统计分析。两种方法的结果表明，每个 DNA 线圈的平均珠数分别约为 6 和 2 个，对于 40nm 和 130nm 珠子的样品分别为 40nm 和 130nm。TEM 分析支持了一个早期的假设，即 40nm 珠子优选地固定在 DNA 线圈的内部，而 130nm 珠子在更大程度上固定在线圈的外部。本工作中展示的方法学应该为研究各种功能化纳米粒子与大分子相互作用提供新的可能性，这对于更深入地了解此类相互作用以及优化许多生物传感器应用都非常有用。
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