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用于体积放大磁性纳米颗粒检测测定的核酸扩增策略。

Nucleic acid amplification strategies for volume-amplified magnetic nanoparticle detection assay.

作者信息

Huang Zhongchao, Li Jing, Zhong Hongwen, Tian Bo

机构信息

Department of Biomedical Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.

出版信息

Front Bioeng Biotechnol. 2022 Aug 10;10:939807. doi: 10.3389/fbioe.2022.939807. eCollection 2022.

DOI:10.3389/fbioe.2022.939807
PMID:36032733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9399362/
Abstract

Magnetic nanoparticles (MNPs) can be quantified based on their magnetic relaxation properties by volumetric magnetic biosensing strategies, for example, alternating current susceptometry. Volume-amplified magnetic nanoparticle detection assays (VAMNDAs) employ analyte-initiated nucleic acid amplification (NAA) reactions to increase the hydrodynamic size of MNP labels for magnetic sensing, achieving attomolar to picomolar detection limits. VAMNDAs offer rapid and user-friendly analysis of nucleic acid targets but present inherence defects determined by the chosen amplification reactions and sensing principles. In this mini-review, we summarize more than 30 VAMNDA publications and classify their detection models for NAA-induced MNP size increases, highlighting the performances of different linear, cascade, and exponential NAA strategies. For some NAA strategies that have not yet been reported in VAMNDA, we predicted their performances based on the reaction kinetics and feasible detection models. Finally, challenges and perspectives are given, which may hopefully inspire and guide future VAMNDA studies.

摘要

磁性纳米颗粒(MNPs)可通过体积磁生物传感策略,例如交流磁化率测量法,基于其磁弛豫特性进行定量分析。体积放大磁性纳米颗粒检测分析方法(VAMNDAs)利用分析物引发的核酸扩增(NAA)反应来增大用于磁传感的MNP标记物的流体动力学尺寸,实现了阿托摩尔至皮摩尔的检测限。VAMNDAs为核酸靶标的分析提供了快速且用户友好的方法,但由于所选择的扩增反应和传感原理而存在固有缺陷。在本综述中,我们总结了30多篇关于VAMNDAs的出版物,并对它们用于NAA诱导的MNP尺寸增加的检测模型进行了分类,突出了不同线性、级联和指数NAA策略的性能。对于一些尚未在VAMNDAs中报道的NAA策略,我们基于反应动力学和可行的检测模型预测了它们的性能。最后给出了挑战和展望,有望为未来的VAMNDAs研究提供启发和指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8590/9399362/a1b0f56e245b/fbioe-10-939807-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8590/9399362/d289fcce7d28/fbioe-10-939807-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8590/9399362/a1b0f56e245b/fbioe-10-939807-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8590/9399362/d289fcce7d28/fbioe-10-939807-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8590/9399362/a1b0f56e245b/fbioe-10-939807-g002.jpg

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