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传统霉菌分析与基于 DNA 的霉菌分析方法比较。

Traditional mould analysis compared to a DNA-based method of mould analysis.

机构信息

United States Environmental Protection Agency, Cincinnati OH, USA.

出版信息

Crit Rev Microbiol. 2011 Feb;37(1):15-24. doi: 10.3109/1040841X.2010.506177. Epub 2010 Sep 29.

Abstract

Traditional environmental mould analysis is based on microscopic observations and counting of mould structures collected from the air on a sticky surface or culturing of moulds on growth media for identification and quantification. These approaches have significant limitations. A DNA-based method of mould analysis called mould specific quantitative PCR (MSQPCR) was created for more than 100 moulds. Based on a national sampling and analysis by MSQPCR of dust in US homes, a scale for comparing the mould burden in homes was created called the Environmental Relative Mouldiness Index (ERMI).

摘要

传统的环境霉菌分析基于对在粘性表面上收集的空气中的霉菌结构进行微观观察和计数,或者在生长培养基上培养霉菌以进行鉴定和定量。这些方法有很大的局限性。为了对 100 多种霉菌进行分析,创建了一种基于 DNA 的霉菌分析方法,称为霉菌特异性定量 PCR(MSQPCR)。基于通过 MSQPCR 对美国家庭灰尘进行的全国性抽样和分析,创建了一个用于比较家庭中霉菌负担的尺度,称为环境相对霉变指数(ERMI)。

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