Bellanger A-P, Reboux G, Roussel S, Grenouillet F, Didier-Scherer E, Dalphin J-C, Millon L
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Besançon, France.
Lett Appl Microbiol. 2009 Aug;49(2):260-6. doi: 10.1111/j.1472-765X.2009.02653.x. Epub 2009 May 27.
The aim of our study was to compare, using real-time (Rt) PCR, quantitative levels of five fungal species in three kinds of dwellings.
Three groups of homes were recruited: moisture-damaged homes (MDH, n = 30), allergic patient homes (APH, n = 25) and paired control homes (CH, n = 55). Five moulds with allergenic compounds or mycotoxin production characteristics (Cladosporium sphaerospermum, Penicillium chrysogenum, Aspergillus versicolor, Alternaria alternata and Stachybotrys chartarum) were quantified using Rt-PCR. Cycle threshold results were expressed in spore equivalent per volume or surface unit using a direct calculation based on a spore standard curve. MDH presented significantly higher amounts of DNA from C. sphaerospermum in both air and surface samples than CH (P < 0.001). APH presented slightly elevated amounts of DNA from A. versicolor in both air and surface samples, compared to CH (P < 0.05).
Rt-PCR quantification of targeted fungal species is a rapid, reliable tool that could be included in a global indoor mould evaluation.
Quantification of C. sphaerospermum using Rt-PCR can help to better target social service intervention in MDH. Quantification of A. versicolor DNA could be informative for characterization of APH.
我们研究的目的是使用实时（Rt）PCR比较三种住宅中五种真菌的定量水平。
招募了三组房屋：受潮损坏房屋（MDH，n = 30）、过敏患者房屋（APH，n = 25）和配对对照房屋（CH，n = 55）。使用Rt-PCR对五种具有致敏化合物或产霉菌毒素特性的霉菌（球形枝孢菌、产黄青霉、杂色曲霉、链格孢菌和黑葡萄穗霉）进行定量。循环阈值结果以每体积或表面单位的孢子当量表示，通过基于孢子标准曲线的直接计算得出。MDH的空气和表面样本中球形枝孢菌的DNA含量均显著高于CH（P < 0.001）。与CH相比，APH的空气和表面样本中杂色曲霉的DNA含量略有升高（P < 0.05）。
对目标真菌物种进行Rt-PCR定量是一种快速、可靠的工具，可纳入全球室内霉菌评估。
使用Rt-PCR对球形枝孢菌进行定量有助于更好地确定MDH中的社会服务干预目标。对杂色曲霉DNA进行定量可能有助于对APH进行特征描述。
