School of Medical Science and Laboratory Medicine, Key Institute of Clinical Laboratory Science, Jiangsu University, Zhenjiang Key Institute of Clinical Laboratory Medicine, 301 Xuefu Road, Zhenjiang City, Jiangsu Province 212013, Peoples Republic of China.
Cell Biol Int. 2011 Feb;35(2):119-23. doi: 10.1042/CBI20100132.
In the present study, MSCs (mesenchymal stem cells) were successfully isolated and identified from hUCC (human uterine cervix cancer) tissues. The morphological appearance, immunophenotype, growth curve, cell cycle, cytogenetic features and differentiation potential of these cells were investigated. Results showed that cells isolated from the uterine cervix cancer tissues displayed fibroblast-like morphology and grew into colonies. Immunophenotyping by flow cytometry revealed that the isolated cells were positive for CD13, CD29, CD44, CD105 and HLA-I, while negative for CD10, CD14, CD31, CD34, CD38 and HLA-DR. The cells kept a normal karyotype by chromosome analysis. At the third passage, the percentages of cells in G0-/G1-, 2-/M- and S-phase were 84.94, 8.36 and 6.71%, respectively. Under appropriate induction conditions, these cells can differentiate into osteogenic, adipogenic cells and hepatocytes. Taken together, MSCs were confirmed to exist in hUCC tissues, which may provide a new target for clinical cancer therapy.
在本研究中，我们成功地从 hUCC（人子宫颈癌）组织中分离和鉴定了间充质干细胞。我们对这些细胞的形态外观、免疫表型、生长曲线、细胞周期、细胞遗传学特征和分化潜能进行了研究。结果表明，从子宫颈癌组织中分离的细胞呈成纤维细胞样形态，并形成集落。通过流式细胞术进行免疫表型分析显示，分离的细胞对 CD13、CD29、CD44、CD105 和 HLA-I 呈阳性，而对 CD10、CD14、CD31、CD34、CD38 和 HLA-DR 呈阴性。染色体分析显示，这些细胞保持正常核型。在第 3 代时，G0/G1 期、2/M 期和 S 期细胞的比例分别为 84.94%、8.36%和 6.71%。在适当的诱导条件下，这些细胞可分化为成骨细胞、脂肪细胞和肝细胞。综上所述，hUCC 组织中存在间充质干细胞，这可能为临床癌症治疗提供新的靶点。
