Division of Urology, Department of Surgery, Chang Gung Medical Foundation, Chia-Yi, Taiwan.
BJU Int. 2011 Jun;107(11):1839-43. doi: 10.1111/j.1464-410X.2010.09597.x. Epub 2010 Sep 28.
What's known on the subject? and What does the study add? It has been known that there is an increase of oxidative damage in the bladder tissues of animals after PBOO. However, no reliable oxidative stress biomarkers in either urine or plasma have been available for the assessment of the severity of PBOO. This study clearly demonstrated that the levels of oxidative stress biomarkers are increased in urine and plasma of the rabbits with PBOO.
To investigate oxidative stress and oxidative damage biomarkers in urine and plasma after partial bladder outlet obstruction (PBOO) in rabbits.
In all, 16 male New Zealand White rabbits were separated equally into four groups: a control group and PBOO-treated groups for 2, 4 and 8 weeks. The oxidative stress biomarkers assessed included urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and plasma malondialdehyde (MDA). We also measured the total antioxidant capacity (TAC) in blood plasma. 8-OHdG, MDA and TAC were measured at both the beginning and indicated time points of the experimental design.
There was no significant difference in body weight among rabbits in the four groups. However, there was a significant increase in bladder weight after 2 weeks of PBOO. After 4 and 8 weeks of PBOO, there was an additional significant increase in bladder weight in all three groups. There was no difference in blood creatinine levels among the groups. In the 4- and 8-week PBOO groups, there was a significant increase of 8-OHdG in urine and of MDA in plasma, while there was a significant decrease in TAC in plasma.
The results showed that oxidative stress could be detected in the plasma and urine of rabbits after 4 and 8 weeks of PBOO, and not only from bladder tissue as previously reported. Thus, there could be an easy and alternative way to evaluate bladder function by analysis of urine and/or plasma. Additionally, rabbits with chronic PBOO showed an increase in systemic oxidative stress, which could be a novel starting point for examining the link between the lower urinary tract symptoms/benign prostate hyperplasia and metabolic syndrome in future studies.
研究兔部分膀胱出口梗阻（PBOO）后尿液和血浆中氧化应激和氧化损伤生物标志物。
16 只雄性新西兰白兔被平均分为 4 组：对照组和 PBOO 处理组（2、4 和 8 周）。评估的氧化应激生物标志物包括尿液 8-羟基-2'-脱氧鸟苷（8-OHdG）和血浆丙二醛（MDA）。我们还测量了血液血浆中的总抗氧化能力（TAC）。在实验设计的开始和指定时间点测量 8-OHdG、MDA 和 TAC。
4 组兔子的体重无显著差异。然而，PBOO 2 周后膀胱重量显著增加。PBOO 4 和 8 周后，三组膀胱重量均有进一步显著增加。各组间血肌酐水平无差异。在 4 周和 8 周的 PBOO 组中，尿液中的 8-OHdG 和血浆中的 MDA 显著增加，而血浆中的 TAC 显著降低。
结果表明，4 周和 8 周的 PBOO 后，兔的血浆和尿液中可检测到氧化应激，而不仅仅是以前报道的膀胱组织。因此，通过尿液和/或血浆分析，可能有更简单的替代方法来评估膀胱功能。此外，慢性 PBOO 的兔子表现出系统性氧化应激增加，这可能是未来研究下尿路症状/良性前列腺增生和代谢综合征之间联系的新起点。
