Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho - 73, 041 81 Kosice, Slovakia.
Dev Comp Immunol. 2011 Feb;35(2):182-92. doi: 10.1016/j.dci.2010.09.008. Epub 2010 Oct 15.
One of the major roles of innate immunity system is the recognition and the determination of the nature of the antigen. This ability is encompassed by specific receptors as Toll-like receptors (TLRs). TLR9 recognizes bacterial and viral CpG motifs, while their potent immunostimulation effect seems to be promising for lentiviral therapies. Recent studies, however, show the presence of a big polymorphism within the TLR genes and the linkage between substitutions and susceptibility to various infections. Moreover, different recognition ability seems to be utilized by different species and possibly breeds. In this study, we characterized the protein coding region of ovine TLR9 gene. By using comparative analysis of two closely related species and humans, we suggest, which characteristics of protein could be responsible for altered recognition. Furthermore, analyzing the presence of the substitutions, we show the intraspecies polymorphism and its possible implications, while attempting to define the association of discovered substitutions with the maedi visna infection.
先天免疫系统的主要作用之一是识别和确定抗原的性质。这种能力被特定的受体所包含，如 Toll 样受体（TLRs）。TLR9 识别细菌和病毒的 CpG 基序，而它们强大的免疫刺激作用似乎对慢病毒治疗有很大的帮助。然而，最近的研究表明，TLR 基因中存在着很大的多态性，并且取代与易感性之间存在着联系。此外，不同的识别能力似乎被不同的物种和品种所利用。在这项研究中，我们对绵羊 TLR9 基因的编码区进行了特征描述。通过对两个密切相关的物种和人类进行比较分析，我们提出了哪些蛋白质特征可能导致识别的改变。此外，通过分析取代的存在，我们展示了种内多态性及其可能的影响，同时试图确定发现的取代与梅迪-维斯纳感染的关联。
