绵羊 Toll 样受体 9（TLR9）多态性 G520R 与小反刍兽疫病毒血清阳性有关。
Toll like receptor 9 (TLR9) polymorphism G520R in sheep is associated with seropositivity for Small Ruminant Lentivirus.
机构信息
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
出版信息
PLoS One. 2013 May 15;8(5):e63901. doi: 10.1371/journal.pone.0063901. Print 2013.
Infectious diseases of sheep are of major economic importance causing direct and indirect losses. Among the major sheep infectious agents are Small Ruminant Lentivirus, Chlamydophila abortus and Mycobacterium avium subsp. paratuberculosis infections, mainly due to their worldwide distribution and economic impact that they cause. Based on the differential susceptibility to infectious diseases between and within breeds and on the recent findings regarding the putative involvement of TLR9 in disease susceptibility, the aim of this study was to evaluate the levels of nucleotide variation of TLR9 and its mediator MyD88 in three sheep flocks originated from different breeds and assess their possible association with seropositivity/seronegativity for different infectious agents. The analysis indicated that the change of G to R at codon 520 of TLR9 polypeptide shows a significant association with Small Ruminant Lentivirus seropositivity. This amino-acid substitution, which can result in polarity change, might influence structure and function of LRR17, interfering with ligand binding and thus could be used in studies investigating susceptibility/resistance to Small Ruminant Lentivirus infections in sheep.
绵羊传染病具有重大的经济重要性，造成直接和间接的损失。在主要的绵羊传染病病原体中，小反刍兽瘟病毒、衣原体流产和分枝杆菌副结核病感染主要是由于其在世界范围内的分布和造成的经济影响。基于不同品种和品种内对传染病的不同敏感性，以及最近关于 TLR9 在疾病易感性中的假定参与的发现，本研究旨在评估来自不同品种的三个绵羊群中 TLR9 及其介体 MyD88 的核苷酸变异水平，并评估它们与针对不同传染病病原体的血清阳性/阴性的可能相关性。分析表明，TLR9 多肽 520 位密码子从 G 到 R 的变化与小反刍兽瘟病毒血清阳性显著相关。这种可能导致极性变化的氨基酸取代可能会影响 LRR17 的结构和功能，干扰配体结合，因此可用于研究绵羊对小反刍兽瘟病毒感染的易感性/抗性。
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