Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n 15782, Santiago de Compostela, Spain.
Talanta. 2010 Oct 15;82(5):1668-73. doi: 10.1016/j.talanta.2010.07.043. Epub 2010 Jul 24.
Raw edible seaweed harvested in the Galician coast (Northwestern Spain), including two red seaweed types (Dulse and Nori), three brown seaweed (Kombu, Wakame and Sea Spaghetti), one green seaweed (Sea Lettuce) and one microalgae (Spirulina platensis) were studied to assess trace elements bioavailability using an in vitro method (simulated gastric and intestinal digestion/dialysis). Similarly, a cooked seaweed sample (canned in brine) consisting of a mixture of two brown seaweed (Sea Spaghetti and Furbelows) and a derived product (Agar-Agar) from the red seaweed Gelidiumm sesquipedale, were also included in the study. The total trace element content as well as the non-dialyzable fractions was carried out after a microwave acid digestion of the seaweed samples by inductively coupled plasma-mass spectrometry (ICP-MS). The dialyzable fraction was determined without any pre-treatment by ICP-MS. PIPES buffer solution at a pH of 7.0 and dialysis membranes of 10kDa molecular weight cut off (MWCO) were used for intestinal digestion. Accuracy of the method was assessed by analyzing a NIES-09 certified reference material (Sargasso seaweed). The accuracy of the in vitro procedure was established by a mass balance study which led to good accuracy of the whole in vitro process, after statistical evaluation (95% confidence interval). The highest dialyzability percentages (100±0.2%) were obtained for Dulse in Mn and V.
在西班牙西北部的加利西亚海岸收获的生食用海藻（包括两种红藻（Dulse 和 Nori），三种褐藻（Kombu、Wakame 和 Sea Spaghetti），一种绿藻（Sea Lettuce）和一种微藻（Spirulina platensis））被用于评估痕量元素的生物利用度采用体外方法（模拟胃和肠消化/透析）。同样，也包括一种煮熟的海藻样品（用盐水罐装），由两种褐藻（Sea Spaghetti 和 Furbelows）和一种红藻 Gelidiumm sesquipedale 的衍生产品（Agar-Agar）混合而成。通过微波酸消解海藻样品，用电感耦合等离子体质谱法（ICP-MS）进行总痕量元素含量和不可透析分数的测定。可透析分数在没有任何预处理的情况下通过 ICP-MS 进行测定。采用 pH 值为 7.0 的 PIPES 缓冲溶液和 10kDa 分子量截止（MWCO）的透析膜进行肠消化。通过分析 NIES-09 认证参考物质（马尾藻海海藻）来评估方法的准确性。通过质量平衡研究建立了体外程序的准确性，经过统计评估（95%置信区间），整个体外过程的准确性良好。Dulse 中 Mn 和 V 的可透析百分比最高（100±0.2%）。
