Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Theriogenology. 2011 Jan 1;75(1):155-63. doi: 10.1016/j.theriogenology.2010.08.001. Epub 2010 Sep 27.
A novel somatostatin (SS) DNA vaccine (pGM-CSF/SS), delivered orally by attenuated Salmonella typhimurium (CSO22), was used to immunize female mice at 5, 7, and 11 wk of age; the objective was to investigate the humoral immune response and effects of this vaccine on growth, reproduction and lactation. The pGM-CSF/SS induced SS-specific antibodies, which peaked (3.69 ± 0.89; mean ± S.D) 4 wk after the first booster immunization. Compared with a saline-treated control group, body weight gain of a pGM-CSF/SS immunized group increased 30.3% (23.88 vs. 18.32 g, P < 0.05) during the growth period (from 2 wk after primary immunization to 4 wk after the first booster immunization). Immunized mice had higher plasma estradiol concentrations (84.10 ± 2.16 vs. 81.45 ± 2.12 pg/ml, P < 0.05) and a shorter estrous cycle (4.06 ± 0.75 vs. 5.33 ± 0.49 d, P < 0.05), but serum progesterone concentrations were not significantly affected. Since offspring produced by immunized mice gained weight faster (P < 0.05) in the first 2 wk of life (4.27 ± 0.62 and 7.81 ± 1.30 g in Weeks 1 and 2, respectively vs. 3.70 ± 0.23 and 7.14 ± 0.48 g), we inferred that pGM-CSF/SS could have a direct or indirect role in regulating lactation in mice. In conclusion, GM-CSF and CSO22 served as adjuvant and attenuated live vector, respectively, with efficient oral delivery of an SS DNA vaccine which successfully induced a humoral immune response and enhanced rate of weight gain. Furthermore, the DNA vaccine pGM-CSF/SS affected plasma estradiol concentrations and the estrous cycle, and seemed to enhance lactation performance of female mice.
一种新型的生长抑素（SS）DNA 疫苗（pGM-CSF/SS），通过减毒鼠伤寒沙门氏菌（CSO22）经口给药，用于免疫 5、7 和 11 周龄的雌性小鼠；目的是研究该疫苗的体液免疫反应及其对生长、繁殖和泌乳的影响。pGM-CSF/SS 诱导产生 SS 特异性抗体，首次加强免疫后 4 周达到峰值（3.69±0.89；平均值±标准差）。与生理盐水处理的对照组相比，pGM-CSF/SS 免疫组的体重增加 30.3%（23.88 与 18.32g，P<0.05），在生长期（从初次免疫后 2 周到首次加强免疫后 4 周）。免疫小鼠的血浆雌二醇浓度更高（84.10±2.16 与 81.45±2.12pg/ml，P<0.05），发情周期更短（4.06±0.75 与 5.33±0.49d，P<0.05），但血清孕激素浓度没有显著影响。由于免疫小鼠的后代在生命的前 2 周体重增加更快（P<0.05）（第 1 周和第 2 周分别为 4.27±0.62 和 7.81±1.30g，而 3.70±0.23 和 7.14±0.48g），我们推断 pGM-CSF/SS 可能在调节小鼠泌乳方面具有直接或间接作用。总之，GM-CSF 和 CSO22 分别作为佐剂和减毒活载体，通过有效的口服途径传递 SS DNA 疫苗，成功诱导了体液免疫反应并提高了体重增加率。此外，DNA 疫苗 pGM-CSF/SS 影响血浆雌二醇浓度和发情周期，并似乎增强了雌性小鼠的泌乳性能。
