College of Animal Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Mol Biol Rep. 2012 Jun;39(6):6773-9. doi: 10.1007/s11033-012-1502-6. Epub 2012 Feb 4.
Female BALB/c mice were actively immunized subcutaneously with a recombinant protein of granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with somatostatin (SS) (GM-CSF/SS). Fifty-four days after the primary immunization, the body weight of the immunized mice increased by 4.62% compared with the control (P < 0.05), together with the induction of detectable serum antibodies against SS. The level of serum growth hormone (GH) elevated by 44.54% (P < 0.05) and the mRNA expression of muscular IGF-1 increased by 94% for the GM-CSF/SS-treated mice. The results indicated that the recombinant protein GM-CSF/SS was efficient in inducing specific immunity against SS, subsequently leading to the increase of the GH level by SS neutralization, and ultimately improving the growth of mice.
雌性 BALB/c 小鼠经皮下主动免疫粒细胞-巨噬细胞集落刺激因子（GM-CSF）与生长抑素（SS）融合重组蛋白（GM-CSF/SS）。初次免疫 54 天后，与对照组相比，免疫小鼠的体重增加了 4.62%（P<0.05），同时诱导出针对 SS 的可检测血清抗体。GM-CSF/SS 处理的小鼠血清生长激素（GH）水平升高了 44.54%（P<0.05），肌肉 IGF-1 的 mRNA 表达增加了 94%。结果表明，重组蛋白 GM-CSF/SS 能有效诱导针对 SS 的特异性免疫，进而通过 SS 中和作用提高 GH 水平，最终促进小鼠生长。
