Akiyama Y, Kazama M, Tahara C, Shimazu C, Otake J, Kamei K, Nakatake T, Sakurai N, Yasumuro Y, Suzuki S
Central Clinical Laboratory, Tokyo University Hospital, Japan.
Thromb Res. 1990 Nov 15;60(4):281-9. doi: 10.1016/0049-3848(90)90107-n.
The circadian fluctuation of hemostasis related parameters was examined on 16 healthy Japanese adults (male 9, female 7). Twenty one parameters were measured in this study, i.e. fibrinogen, the activity of F.II, F.V., F.VII, F.VIII, F.IX, F.X., F.XI, F.XII, antithrombin III, plasminogen, alpha 2-antiplasmin, as well as the antigen level of F.IX, von Willebrand Factor, protein C, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), beta-thromboglobulin, platelet factor 4, fibrinopeptide A, plasmin-alpha 2-antiplasmin complex and FDP. Fluctuation was not significant in almost all of the parameters except F.VIII, F.IX, beta-thromboglobulin, platelet factor 4, tPA and PAI-1. Although the fluctuations of F.VIII, F.IX, beta-thromboglobulin and platelet factor 4 were statistically significant, they remained within the normal ranges. On the other hand, tPA and free PAI-1 showed significant circadian fluctuation, of which levels were highest at 9:00. It was postulated that the significant circadian fluctuation of fibrinolytic activity will be regulated by the balance between tPA and PAI-1 in plasma.
对16名健康日本成年人（男性9名，女性7名）的止血相关参数的昼夜波动情况进行了检测。本研究测量了21项参数，即纤维蛋白原、凝血因子II、V、VII、VIII、IX、X、XI、XII的活性、抗凝血酶III、纤溶酶原、α2 - 抗纤溶酶，以及凝血因子IX、血管性血友病因子、蛋白C、组织型纤溶酶原激活物（tPA）、纤溶酶原激活物抑制剂 - 1（PAI - 1）、β - 血小板球蛋白、血小板第4因子、纤维蛋白肽A、纤溶酶 - α2 - 抗纤溶酶复合物和纤维蛋白降解产物的抗原水平。除了凝血因子VIII、IX、β - 血小板球蛋白、血小板第4因子、tPA和PAI - 1外，几乎所有参数的波动均不显著。虽然凝血因子VIII、IX、β - 血小板球蛋白和血小板第4因子的波动具有统计学意义，但仍在正常范围内。另一方面，tPA和游离PAI - 1表现出显著的昼夜波动，其水平在9:00时最高。据推测，纤溶活性的显著昼夜波动将受血浆中tPA和PAI - 1之间平衡的调节。
