Biomedical Engineering Institute, South China University of Technology, Guangzhou 510640, People's Republic of China.
Biomed Mater. 2010 Oct;5(5):054103. doi: 10.1088/1748-6041/5/5/054103. Epub 2010 Sep 28.
Sol-gel-derived bioactive glasses (BGs) have been developed for bone tissue regeneration. To develop more reliable bone tissue repair systems, it is necessary to control the morphology and surface textures of bioactive glasses. In this study, we prepared bioactive glasses by sol-gel technology using hydrochloride acid, lactic acid, citric acid and acetic acid as hydrolysis catalysts. We studied effects of acids on the morphology and surface textures, apatite-forming bioactivity and cellular response (cellular attachment and proliferation) of BGs. Results showed that the surface morphology, structure, apatite-forming bioactivity and cellular response of BG particles can be controlled by changing acid species. The hydrochloric acid-derived bioactive glass (HBG) and the acetic acid-derived bioactive glass (ABG) present high surface areas and fast apatite-forming rates. Lactic acid- and citric acid-derived bioactive glasses (LBG, CBG) exhibited nanoscale surface morphology, relatively low surface areas and comparable apatite-forming bioactivity. The results of human marrow mesenchymal stem cell (HMSC) culture exhibited that LBG and CBG have an enhanced effect on the cell proliferation, as compared to HBG, ABG and tissue culture plate. This study suggests that sol-gel bioactive glasses with proper surface textures and apatite-forming rate can affect preliminary cellular proliferation.
溶胶-凝胶法衍生的生物活性玻璃（BGs）已被开发用于骨组织再生。为了开发更可靠的骨组织修复系统，有必要控制生物活性玻璃的形态和表面纹理。在这项研究中，我们使用盐酸、乳酸、柠檬酸和乙酸作为水解催化剂，通过溶胶-凝胶技术制备了生物活性玻璃。我们研究了酸对 BG 形态、表面纹理、磷灰石形成生物活性和细胞反应（细胞附着和增殖）的影响。结果表明，通过改变酸的种类可以控制 BG 颗粒的表面形貌、结构、磷灰石形成生物活性和细胞反应。盐酸衍生的生物活性玻璃（HBG）和乙酸衍生的生物活性玻璃（ABG）具有高的比表面积和快速的磷灰石形成速率。乳酸和柠檬酸衍生的生物活性玻璃（LBG、CBG）具有纳米级表面形貌、相对较低的比表面积和可比的磷灰石形成生物活性。人骨髓间充质干细胞（HMSC）培养的结果表明，与 HBG、ABG 和组织培养板相比，LBG 和 CBG 对细胞增殖具有增强作用。本研究表明，具有适当表面纹理和磷灰石形成速率的溶胶-凝胶生物活性玻璃可以影响初步的细胞增殖。
