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Fidelity of DNA polymerase alpha partially purified from a mutator mutant and wild-type mouse FM3A cells.

作者信息

Hyodo M, Suzuki K

机构信息

Department of Molecular Biology, School of Medicine, Tokai University, Kanagawa, Japan.

出版信息

Tokai J Exp Clin Med. 1990 Mar;15(1):5-11.

PMID:2087706
Abstract

Several mutator mutants were isolated from a cultured mouse cell line (FM3A). The mutants exhibited a high rate of spontaneous mutation at three genetic loci of drug resistance. To investigate a possible link between mutator phenotype and fidelity of DNA replication, DNA polymerase alpha was partially purified from the wild-type and the mutator mutant (Fmut 1) showing the highest mutation rate. Using a combination of synthetic template and primer, the ratio of incorporation of incorrect to correct nucleotides was determined. The results indicated that the DNA polymerase alpha from the mutator mutant showed a slightly higher rate of misincorporation, 1.4 and 1.6 times, than that of the wild-type.

摘要

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